|
|||||
|
|
| 油菜分子标记图谱构建及抗菌核病性状的QTL定位 | |
| 引用本文： | 刘春林,官春云,李栒,阮颖,廖晓兰,熊兴华,周小云,王国槐,陈社员.油菜分子标记图谱构建及抗菌核病性状的QTL定位[J].遗传学报,2000,27(10):918-924. |
| 作者姓名： | 刘春林 官春云 李栒 阮颖 廖晓兰 熊兴华 周小云 王国槐 陈社员 |
| 作者单位： | 1. 湖南农业大学作物基因工程湖南省重点实验室，长沙 410128 2. 湖南农业大学理学院，长沙 410128 3. 湖南农业大学植物科技学院，长沙 410128 |
| 摘 要： | 以油菜品种０８５（抗菌核病）与湘油１３号杂交得到的Ｆ２代作为作图群体，以ＲＡＰＤ标记来构建油菜连锁图谱。通过对３００个１０－ｍｅｒ随机引物的筛选，共获得２００个多态性ＲＡＰＤ位点。经Ｍａｐｍａｋｅｒ／ＥＸＰ３．０软件处理，构建了１张含１９３个标记位点、１９个连锁群、覆盖长度为１３２４ｃＭ的连锁图谱。在此基础上利用Ｍａｐｍａｋｅｒ／ＱＴＬ １．１将抗菌核病基因定位在第四、八和十四３个连锁群上，即：Ｓ |
| 关 键 词： | 甘蓝型油菜 连锁图构建 抗菌核病性状 QTL定位 |
| 文章编号： | 0379-4172（2000）10-0918-07 |
| 修稿时间： | 2000年1月6日 |
Construction of Linkage Map and Mapping Resistance Gene of Scterotinia scterotiorum in Brassica napus |
|
| LIU Chun-lin,GUAN Chun-Yun,LI Xun,RUAN Ying,LIAO Xiao-Lan,XIONG Xing-hua,ZHOU Xiao-Yun,WANG Guo-huai,CHEN She-yuan.Construction of Linkage Map and Mapping Resistance Gene of Scterotinia scterotiorum in Brassica napus[J].Journal of Genetics and Genomics,2000,27(10):918-924. | |
| Authors: | LIU Chun-lin GUAN Chun-Yun LI Xun RUAN Ying LIAO Xiao-Lan XIONG Xing-hua ZHOU Xiao-Yun WANG Guo-huai CHEN She-yuan |
| Abstract: | Scterotinia scterotiorum (Lib.) de by is a world-wide disease, which seriously decreases the yield and the quality of oil of rapeseed. Mapping resistance genes of S. scterotiorum is of vital effect to breed new elite cultivars resisting the disease. In this paper, we reported that a RAPD linkage map was constructed, including 193 RAPD markers, 19 linkage groups and covering 1 324 cM, by using Mapmaker 3.0. On the basis of map, and resistance genes, Sc1, Sc2 and Sc3, were located in 4th, 8th and 18th linkage groups. Among three QTLs, Sc2 has the characteristics of major gene with explained 29%. The three QTLs together explained about 56% of phenotypic variation. |
| Keywords: | Brassica napus linkage groups S scterotiorum resistance character QTL mapping |
| 本文献已被 维普 万方数据 等数据库收录！ | |