BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究

以控制BooroolaMerino羊多胎性能的BMPR IB基因 ,以及影响Invedale和Hanna羊排卵数的BMP15基因作为候选基因 ,从分子水平上对小尾寒羊的多胎机制进行研究 ,分析突变位点的特性 ,并通过大规模的群体检测统计推断其遗传效应。实验结果表明 :多胎品种小尾寒羊在BMPR IB基因的相应位置上发生了与BooroolaMerino羊相同的突变 (A74 6G) ,该基因的BB基因型在小尾寒羊群体内为优势基因型 ,且小尾寒羊初产和经产母羊的BB基因型比 ++基因型分别多产 0 97羔 (P <0 0 5 )和 1 5羔 (P <0 0 1) ,推测BMPR IB基因与控制小尾寒羊多胎性能的主效基因存在紧密的遗传连锁。而BMP15基因在小尾寒羊中不存在V31D或Q2 3Ter突变 ,说明小尾寒羊的多胎遗传机制与Romney羊不同 ,因此排除了BMP15突变影响小尾寒羊排卵数的可能性。

Studies of BMPR-IB and BMP15 as Candidate Genes for Fecundity in Little Tailed Han Sheep

BMPR-IB gene which controls the fecundity of Booroola Merino and BMP15 gene which affects the ovulation of Invedale and Hanna were studied as candidate genes on the fecundity of Little Tailed Han Sheep,and their mutations and genetic effects were analyzed.The results showed that there was a same mutation in BMPR-IB gene(A746G)of Little Tailed Han Sheep as that of Booroola Merino.The BB mutation genotype was superior in prolific Little Tailed Han Sheep,and the ewes with genotype BB had 0.97( P <0.05)and 1.5( P <0.01)lambs more than those with genotype ++ in the first parity and later parities,respectively.It could be inferred that BMPR-IB gene was related with the major gene that controls the high prolificacy of Little Tailed Han Sheep.While there was not mutation of V31D or Q23Ter in BMP15 gene of Little Tailed Han Sheep,it showed that the fecundity mechanism of Little Tailed Han Sheep was different from that of Romney sheep.Then it was ruled out the possibility that the ovulation of Little Tailed Han Sheep was affected by the mutation of BMP15 gene.