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| 野败型杂交水稻恢复基因的AFLP标记研究 | |
| 引用本文： | 何光华,裴炎,杨光伟,唐梅,谢戎,侯磊,杨正林,李永洪.野败型杂交水稻恢复基因的AFLP标记研究[J].遗传学报,2000,27(4):304-310. |
| 作者姓名： | 何光华 裴炎 杨光伟 唐梅 谢戎 侯磊 杨正林 李永洪 |
| 作者单位： | 1. 西南农业大学生物技术中心,重庆北碚,400716;四川省农业科学院水稻高粱研究所,泸州,646100 2. 西南农业大学生物技术中心,重庆北碚,400716 3. 四川省农业科学院水稻高粱研究所,泸州,646100 |
| 基金项目： | 四川省生物技术重点项目，四川省应用基础研究项目 |
| 摘 要： | 选择汕优６３Ｆ２的高可育株和高不育株分别建立２个基因池，利用ＡＦＬＰ标记技术对２池间的多态性进行了研究，分析表明，６４组引物在两池间全部扩增出了稳定，清晰的带纹，共计３４７７条带。多数引物在基因池间未呈现多态性，只有引物组合Ｅ－ＡＧＣ／Ｍ－ＣＡＡ在基因池间表现多态，用双亲、Ｆ１、Ｆ２单株以及生产和育种上的骨干不育系与恢复系验证均表明这组引物所揭示的多态性片段与恢复基因有关（命名为ＡＰ１）。ＡＰ１为 |
| 关 键 词： | 野败型 杂交水稻 恢复基因 AFLP标记 结实率 |
AFLP Markers of Restoring Genes of the Wild-Abortive Hybrid Rice |
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| HE Guang-Hua,PEI Yan,YANG Guang-Wei,TANG Mei,XIE Rong,HOU Lei,YANG Zheng-Lin,LI Yong-Hong.AFLP Markers of Restoring Genes of the Wild-Abortive Hybrid Rice[J].Journal of Genetics and Genomics,2000,27(4):304-310. | |
| Authors: | HE Guang-Hua PEI Yan YANG Guang-Wei TANG Mei XIE Rong HOU Lei YANG Zheng-Lin LI Yong-Hong |
| Abstract: | Fertile and sterile pools were set up by bulked segregant analysis (BSA) based on the selection for the highly fertile and highly sterile plants of Shanyou 63 F_2 populaiton The AFLP analysis of the two pools indicated that 64 primer combinations amplified 3 477 stable and clear bands. Exception of the combination E-AGC/M-CAA, all primer combinations had not detected polymorphism between the two pools. It was proved by investigation of two parents, individuals of F_2 segregant population, backbone sterile lines and restorer lines that the polymorphic fragment AP_1, generated from the primer E-AGC/M-CAA was associated with the restoring gene. AP_1 was a single copy detected by Southern blot hybridization. The distance between AP_1 and the restoring gene was 4.76cM. |
| Keywords: | wild-abortive hybrid rice restoring gene AFLP marker |
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