Large-Scale Forward Genetic Screening Analysis of Development of Hematopoiesis in Zebrafish |
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| Authors: | Kun Wang Zhibin Huang Lingfeng Zhao Wei Liu Xiaohui Chen Ping Meng Qing Lin Yali Chi Mengchang Xu Ning Ma Yiyue Zhang Wenqing Zhang |
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| Institution: | Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology,School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China |
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| Abstract: | Zebrafish is a powerful model for the investigation of hematopoiesis.In order to isolate novel mutants with hematopoietic defects,large-scale mutagenesis screening of zebrafish was performed.By scoring specific hematopoietic markers,52 mutants were identified and then classified into four types based on specific phenotypic traits.Each mutant represented a putative mutation of a gene regulating the relevant aspect of hematopoiesis,including early macrophage development,early granulopoiesis,embryonic myelopoiesis,and definitive erythropoiesis/lymphopoiesis.Our method should be applicable for other types of genetic screening in zebrafish.In addition,further study of the mutants we identified may help to unveil the molecular basis of hematopoiesis. |
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| Keywords: | Zebrafish Large-scale screening Hematopoiesis |
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