| 普通小麦Ｔ型细胞质雄性不育系及其保持系线粒体ＤＮＡ的比较研究 |
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| 引用本文： | 仇艳光,沈银柱,黄占景,赵保存,何聪芬,秘彩莉.普通小麦Ｔ型细胞质雄性不育系及其保持系线粒体ＤＮＡ的比较研究[J].遗传学报,2001,28(2):166-170. |
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| 作者姓名： | 仇艳光 沈银柱 黄占景 赵保存 何聪芬 秘彩莉 |
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| 作者单位： | 河北师范大学生命科学学院, |
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| 基金项目： | 河北省基金委资助项目（编号：300170） |
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| 摘 要： | 以小麦Ｔ细胞质雄性不育系７５－３３６９Ａ和相应保系７５－３３６９Ｂ为材料，用限制性内切酶ＢamHⅠ、EcoRⅠ、hINDⅢ完全酶解，以Ｏenothera mtDNA qtp6,小麦线粒体基因nad3/rps12、cos1为探针进行Southern杂交，杂交结果表明，７５－３３６９Ａ和 75-3369B在这３个基因上或附近有显著的组织结果差异，推测这些差异可能影响了线粒体基因组的正常功能，最终引起了７５－３３６９Ａ雄性不育。
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| 关 键 词： | 普通小麦 T型细胞质雌性不育 线粒体 分子杂交 |
| 文章编号： | 0379-4172（2001）02-0166-05 |
| 修稿时间： | 2000年5月29日 |
Comparative Studies of
Mitochondrial DNA of T-type Cytoplasmic Male Sterile Lines and Their Maintainers in Wheat |
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| QIU Yan-Guang,SHEN Yin-zhu,HUANG Zhan-jing,ZHAO Bao-Cun,HE Cong-fen,BI Cai-Li.Comparative Studies of
Mitochondrial DNA of T-type Cytoplasmic Male Sterile Lines and Their Maintainers in Wheat[J].Journal of Genetics and Genomics,2001,28(2):166-170. |
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| Authors: | QIU Yan-Guang SHEN Yin-zhu HUANG Zhan-jing ZHAO Bao-Cun HE Cong-fen BI Cai-Li |
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| Abstract: | mtDNAs of T type wheat cytoplasmic male sterile lines 75-369A and their maintainers 75-369B were isolated and digested completely with restriction endonuclease BamH, EcoR, Hind. The mitochondrial genomic difference between CMS line and maintainers were revealed by Southern hybridization with probes of atp6 from Oenothera and nad3 ／ rps12, cox from wheat mitochondria. Southern hybridization showed the differences between these two lines. Discussion was made on the change of the DNA composition and the male sterile cytoplasm. |
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| Keywords: | wheat T-CMS mtDNA Southern blotting |
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