大熊猫繁育障碍与染色体脆性位点的相关性研究

通过研究建立起适合大熊猫染色体脆性位点表达的BrdU诱导体系。以大熊猫外周血淋巴细胞为材料，通过较长时间培养（96h），采用低浓度BrdU(10μg/ml），短时间（4h）诱导，并结合复制带技术将大熊猫染色体脆性位点的高发生区段准确地定位在No.2和No.12号染色体着丝粒区域。经生物统计学分析发现，No.2和No.12号染色体脆性位点表达频率在个体中有明显差异，而且前者与大熊猫个体的子代存活率呈负相关（r=-0.772).研究结果提示,No.2染色体着丝粒处高效表达的脆性位点地大熊猫个体繁育及其后代的存活是不利的.

Studies of the Corelation Between Fragile Sites and Giant Panda's Breeding Problem

An unique culture sustem has been established which can effectively indicate fragile sites induced by BrdU on giant panda chromosomes. After we cultured the lymphocytes of giant panda for 96 hours and treated it with low concentration of BrdU (10ug/ml) for only a short time (4 hours), the areas of high-frequency-expressed fragile sites were successfully located on the centromeres of No. 2 and No. 12 chromosomes. After statistic analysis, we found that the frequency of fragile sites on No.2 and No. 12 chromosomes are notably different among pandas. What is more important the former is relative to giant pandas' breeding conditions and correlation coefficiency (r equals to -0.772). The result indicates that high frequency of fragile sites on No.2 chromosome is unfavorable to giant panda's breeding and survival of filial generation.