利用花粉辐射诱发普通小麦与大赖草染色体易位的研究

将小麦－大赖草Ｌｒ．２和Ｌｒ．１４染色体二体异附加系９４Ｇ１５和９４Ｇ４５即将成熟花粉经＾６０Ｃｏ－γ射线辐射处理，然后分别给普通小麦品种扬麦５号和绵阳１１授粉。辐射Ｍ１的ＰＭＣ ＭＩ期染色体Ｇｉｅｍｓａ Ｃ－分带和基因组ＤＮＡ荧光原位杂交处理后进行染色体配对分析，从１７株Ｍ１单株中筛选到５株ＰＭＣ ＭＩ期大赖草染色体与小麦配对的个体。根据这５株单株自交Ｍ２种子根尖细胞有丝分裂中期染色体Ｃ－分带和

Studies of the Development of Triticum aestivum-Leymus racemosus Translocation Lines by Pollen Irradiation

Pollens of 94G15 and 94G45, two wheat lines added to chromosome Lr. 14 and Lr. 2 of Leymus racemosus respectively were irradiated via ~60Co-r rays and then used to hybridize with two common wheat varieties-Yangmai 5 and Mianyang 11. The five plants showing chromosome pairing between T aestivum and L racemosus at PMC MI stage were selected from seventeen examined plants of M, progeny as the result of meiosis configuration analysis of chromosomes treated with Giemsa C-banding and fluorescent in situ hybridization. Among which, two T aestivum-L racemosus alien translocation lines-LW8(3) 1 and LWI 1(3) 1 were developed on the basis of further identification of M2, RTC chromosomes by C-banding and in situ hybridization. In addition, feasibility and effectiveness of the pollen irradiation in the development of T aestivm-relatives alien translocations and the availability of the translocation lines of T aestivum-L racemosus are also discussed.