蝰科(Viperidae)蝮亚科(Crotalinae)线粒体12S rRBA基因序列分析及其系统发育

对蝮亚科（蛇岛蝮Ｇｌｏｙｄｉｕｓ ｓｈｅｄａｏｅｎｓｉｓ Ｚｈａｏ、黑眉蝮Ｇｌｏｙｄｉｕｓ ｓａｘａｔｉｌｉｓ Ｅｍｅｌｉａｎｏｖ、乌苏 里蝮Ｇｌｏｙｄｉｕｓ ｕｓｓｕｒｒｉｅｎｓｉｓ　 Ｅｍｅｌｉａｎｏｖ、　竹叶青Ｔｒｉｍｅｒｅｓｕｒｕｓ 　ｓｔｅｊｎｅｇｅｒｉ 　Ｓｃｈｍｉｄｔ和分别来自不 同地区的尖吻蝮Ｄｅｉｎａｇｋｉｓｔｒｏｄｏｎ　ａｃｕｔｕｓ　Ｇｕｅｎｔｈｅｒ、短尾蝮Ｇｌｏｙｄｉｕｓ　ｂｒｅｖｉｃａｕｄｕｓ　Ｓｔｅｊｎｅｇｅｒ各 两条）６种蛇共８个个体测定、分析了约３７０ｂｐ线粒体１２Ｓ ｒＲＮＡ基因序列；以游蛇科链蛇属半 棱鳞链蛇Ｄｉｎｏｄｏｎ ｓｅｍｉｃａｒｉｎａｔｕｓ　序列为外群构建分子系统树。分子数据结果支持尖吻蝮形态 学的属级分类地位；提示蛇岛蝮位于黑眉蝮的蛇岛亚种分类地位，同时探讨了蛇岛蝮的起源 问题；并提示短尾蝮和乌苏里蝮同位于种级分类地位。

Phylogenetic Relationships Among Viperidae, Crotalinae Based on Mitochondrial 125 rRNA Sequence Variations

This paper analyzed the phylogenetic relationships and classification of pit vipers (Viperidae, Crotalinae) based on mitochondrial 12S rRNA gene sequence variations. We have sequenced mtDNA 12S rRNA gene about 370bp fragment from Gloydius saxatilis Emelianov, Gloydius shedaoensis Zhao, Gloydius ussurriensis (Emelianov), Trimeresurus stejnegeri Schmidt and Deinagkistrodon acutus, Gloydius brevicaudus (Stejneger) from two different localities, respectively. Combined with the sequence of Dinodon semicarinatus from GenBank, we have constructed two molecular phylogenetic trees using both Maximum-parsimony analysis and Neighbor-joining. Our results support the following conclusions f (1) Deinagkistrodon is a valid genus; (2) we also discussed the origin of Gloydius shedaoensis and showed it is a subspecies of Gloydius saxatilis, (3) Gloydius brevicaudsu and Gloydius ussurriensis are classified two species.