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中国5种珍稀绢蝶非损伤性取样的mtDNA序列及系统进化
引用本文:陈永久,张亚平,沈发荣,张云武,杨大荣,聂龙,杨跃雄.中国5种珍稀绢蝶非损伤性取样的mtDNA序列及系统进化[J].遗传学报,1999(3).
作者姓名:陈永久  张亚平  沈发荣  张云武  杨大荣  聂龙  杨跃雄
基金项目:国家自然科学基金,云南省应用基础研究基金,国家杰出青年科学基金
摘    要:应用非损伤性取样DNA测序技术测定了4种来自云南白马雪山和1种来自新疆天山的5种珍稀绢蝶的线粒体DNA细胞色素b基因部分DNA序列。在获得的433bp的序列中,A+T约占754%,其中40个核苷酸位点存在变异(约924%)。DNA一级序列数据显示,该5种绢蝶间DNA序列变异丰富。PAUP3.1.1(简约法)数据分析软件构建该5个种绢蝶的分子系统树显示,爱珂绢蝶(Parnassiusacco)和巴裔绢蝶(Parnassiusbaileyi)的亲缘关系比较接近,阿波罗绢蝶(Parnassiusapollo)、珍珠绢蝶(Parnassiusorlears)和西猴绢蝶(Parnassiussimo)3种绢蝶均为相对独立的一支,其中西猴绢蝶分化较早,与形态学研究结果相吻合。

关 键 词:绢蝶  非损伤性取样  mtDNA  系统进化

The Phylogeny of 5 Chinese Peculiar Parnassius Butterflies Using Noninvasive Sampling mtDNA Sequences
CHEN Yong-Jiu, ZHANG Ya-Ping, SHEN Fa-Rong, ZHANG Yun-Wu,YANG Da-Rong, NIE Long, YANG Yue-Xiong.The Phylogeny of 5 Chinese Peculiar Parnassius Butterflies Using Noninvasive Sampling mtDNA Sequences[J].Journal of Genetics and Genomics,1999(3).
Authors:CHEN Yong-Jiu  ZHANG Ya-Ping  SHEN Fa-Rong  ZHANG Yun-Wu  YANG Da-Rong  NIE Long  YANG Yue-Xiong
Abstract:This paper employs nonivasive sampling DNA sequencing technique for estimating molecular phylogeny of 5 Chinese peculiar Parnassius Butterflies. Of 433bp mitochondrial cytochrome b DNA sequences, A% T% was 75.4%, and 40 nucleotide sites were substituted (about 9.24%). The molecular phylogenetic tree constructed by parsimony method suggests that Parnassius acco is closely related to Parnassius baileyi, Parnassius apollo, Parnassius orlears, and Parnassius simo are independent branches respectively. Parnassius simo is divergent earlier. The result is identical with that of morphology.
Keywords:Parnassius  Noninvasive sampling  mtDNA  Phylogeny
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