用微卫星标记定位小麦T型CMS的恢复基因

以T型细胞质雄性不育系 75 336 9A×恢复系 72 6 9 10的F2 群体作为育性调查和基因定位群体。通过育性分析 ,确定该恢复系含有 2个主效恢复基因 ;结合群分法 ,对恢复基因进行了SSR分子标记定位 ,在 2 30对微卫星引物中 ,微卫星标记Xgwm136和Xgwm5 5 0分别与 2个主效恢复基因连锁。这两个标记与Rf基因之间的遗传距离分别为 6 7cM和 5 1cM ,从而将该恢复基因定位在 1AS、1BS染色体上。

Location of the Fertility Restorer Gene for T -Type CMS Wheat by Microsatellite Marker

Through the genetic analysis of a F 2 population,derived from CMS line 75-3369A (T-type CMS wheat) and the restorer line 7269-10,the result indicated that the restorer line was conditioned by two dominant genes.A F 2 population was used to map the fertility restorer (Rf) gene by microsatellite and BSA (bulked segregant analysis).Restorer and sterile DNA pools were established using the extreme fertile and sterile plants of F 2 population,respectively.Among the 230 pairs of microsatellite primers,two markers were found polymorphic between the two pools.Linkage analysis showed that microsatellite marker Xgwm136 and Xgwm550 were linked with the two fertility restorer genes,respectively.One of the Rf gene was located on 1AS and the genetic distance between the SSR marker Xgwm136 and this Rf gene was 6.7 cM,the other Rf gene was located on 1BS and with a genetic distance of 5.1cM to marker Xgwm550.