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苏云金芽胞杆菌菌株YBT833含不同杀虫晶体蛋白基因转化子的特性
引用本文:鲁松清,刘子铎,喻子牛.苏云金芽胞杆菌菌株YBT833含不同杀虫晶体蛋白基因转化子的特性[J].遗传学报,2000,27(9):839-844.
作者姓名:鲁松清  刘子铎  喻子牛
作者单位:华中农业大学微生物科学技术系,农业微生物农业部重点实验室,武汉,430070
基金项目:863(101-03-01-01)及九五攻关项目(96-C01-02-01)资助
摘    要:用电脉冲方法将含有苏云金芽胞杆菌杀虫晶体蛋白基因cry1C的重组质粒pBMBLC转入野生菌株YBT833,获得含不同杀虫晶体蛋白基因的4个转化子。质粒检测和Southern杂交证明它们均为菌株YBT833含重组质粒pBMBLC的转化子。PCR扩增表明,转化子YBT833-1保留了原有的杀虫晶体蛋白基因;转化子YBT833-2丢失了基因cry1Ab;转化子YBT833-3则丢失了所有的杀虫晶体蛋白基

关 键 词:苏云金芽胞杆菌  杀虫晶体蛋白基因  质粒消除
文章编号:0379-4172(2000)09-0839-06
修稿时间:2000年1月7日

The Characteristics of Bacillus thuringiensis Strain YBT833 and Its Transformants that Containing Different ICP Genes
LU Song-Qing,LIU Zi-Duo,YU Zi-Niu.The Characteristics of Bacillus thuringiensis Strain YBT833 and Its Transformants that Containing Different ICP Genes[J].Journal of Genetics and Genomics,2000,27(9):839-844.
Authors:LU Song-Qing  LIU Zi-Duo  YU Zi-Niu
Abstract:Four different transformants_were_selected by transferring cry1C into Bacillus thuringiensis strain YBT833. Southern blot and Plasmid profiles results all proved that cry1C was transferred into strain YBT833. However, it was found by PCR analysis that transformant YBT833-1 kept all indigenous ICP(insecticidal crystal protein) genes of strain YBT833 while transformant YBT833-2 lost cry1Ab, and transformant YBT833-3 lost all ICP genes. SDS-PAGE showed that transformants of YBT833-1, YBT833-2 and YBT833 all had 130kDa and 65kDa peptide bands, but transformant YBT833-3 did not have 65kDa peptide band. Bioassay results showed that the toxicities of all transformants to Spodoptera exigua, Heliothis armigera and Plutella xylostella were lower than that of strain YBT833, except the toxicity of transformant YBT833-2 to P. xylostella which was obviously higher than that of YBT833.
Keywords:Bacillus thuringiensis  electroporation  insecticidal crystal protein gene  plasmid curing
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