| The construction of a genetic linkage map of non-heading Chinese cabbage （Brassica campestris ssp. chinensis Makino） |
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| 作者姓名： | Yan Cheng ;Jianfeng Geng ;Jingyi Zhang ;Qian Wang ;Qingyu Ban ;Xilin Hou |
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| 作者单位： | [1]State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; [2]Key laboratory of Southern Vegetable Crop Genetic Improvement, Ministry ofAgriculture, Nanjing Agricultural University, Nanjing 210095, China; [3]Institute of Horticulture, Henan Academy of Agricultural Science, Zhengzhou, 450002, China |
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| 基金项目： | This,work,is,supported,by,the,National,High-tech,R&Dprogram,(863,Program),the,National,Natural,Science,Foundation |
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| 摘 要： | Non-heading Chinese cabbage （Brassica carnpestris ssp. chinensis Makino） is one of the most important vegetables in eastern China. A genetic linkage map was constructed using 127 doubled haploid （DH） lines, and the DH population was derived from a commercial hybrid ＂Hanxiao＂ （lines SW-13 x L-118）. Out of the 614 polyrnorphic markers, 43.49% were not assigned to any of the linkage groups （LGs）. Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios, and the direction of distorted segregation was mainly toward the paternal parent L-118. After sequentially removing the markers that had an interval distance smaller than 1 cM from the upper marker, the overall quality of the linkage map was increased. Two hundred and sixty-eight molecular markers were mapped into 10 LGs, which were anchored to the corresponding chromosome of the B. rapa reference map based on com- mon simple sequence repeat （SSR） markers. The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM. The number of markers on each LG ranged from 18 （R08） to 64 （R07）, with an average interval distance within a single LG from 1.70 cM （R07） to 6.71 cM （R06）. Among these mapped markers, 169 were sequence-related amplified polymorphism （SRAP） molecular markers, 50 were SSR markers and 49 were random amplification polymorphic DNA （RAPD） markers. With further saturation to the LG9 the current map offers a genetic tool for loci analysis for important agronomic traits.
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| 关 键 词： | 卷心菜 甘蓝 连锁图 SRAP SSR RAPD |
| 收稿时间： | 9 November 2008 |
The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp. Chinensis Makino) |
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| Yan Cheng,;Jianfeng Geng,;Jingyi Zhang,;Qian Wang,;Qingyu Ban,;Xilin Hou.The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp. Chinensis Makino)[J].Journal of Genetics and Genomics,2009,36(8):501-508. |
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| Authors: | Yan Cheng Jianfeng Geng Jingyi Zhang Qian Wang Qingyu Ban Xilin Hou |
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| Institution: | 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2. Key laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;3. Institute of Horticulture, Henan Academy of Agricultural Science, Zhengzhou, 450002, China;1. Department of Biology, Utah State University, Logan, UT 84322-5305, USA;2. Utah Water Research Laboratory, Utah State University, Logan, UT 84322-5305, USA;3. Department of Biological Engineering, Utah State University, Logan, UT 84322-5305, USA;1. Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture of China, Beijing 100081, PR China;2. Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Tianjin 300191, PR China;1. Department of Ecology, Environment and Plant Sciences, Stockholm University, 10654 Stockholm, Sweden;2. Department of Marine Sciences, University of Gothenburg, 40530 Gothenburg, Sweden;1. Laboratory of Brain Function and Disease in Chinese Medicine, College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China;2. The Affiliated Children''s Hospital, College of Medical Sciences, Zhejiang University, Hangzhou, Zhejiang, China;3. Department of Pharmacology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, College of Pharmaceutical Sciences, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China;1. Department Science of Health, School of Medicine and Surgery, University of Catanzaro, Italy;2. Oasi Institute for Research on Mental Retardation and Brain Aging (IRCCS), Troina, EN, Italy |
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| Abstract: | Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is one of the most important vegetables in eastern China.A genetic linkage map was constructed using 127 doubled haploid (DH) lines, and the DH population was derived from a commercial hybrid "Hanxiao" (lines SW-13 × L-118). Out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups (LGs). Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios, and the direction of distorted segregation was mainly toward the paternal parent L- 118. After sequentially removing the markers that had an interval distance smaller than 1cM from the upper marker, the overall quality of the linkage map was increased. Two hundred and sixty-eight molecular markers were mapped into 10 LGs, which were anchored to the corresponding chromosome of the B. rapa reference map based on common simple sequence repeat (SSR) markers. The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM. The number of markers on each LG ranged from 18 (R08) to 64 (R07), with an average interval distance within a single LGfrom 1.70 cM (R07) to 6.71 cM (R06). Among these mapped markers, 169 were sequence-related amplified polymorphism (SRAP) molecular markers, 50 were SSR markers and 49 were random amplification polymorphic DNA (RAPD) markers. With further saturation to the LG, the current map offers a genetic tool for loci analysis for important agronomic traits. |
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| Keywords: | non-heeding Chinese cabbage genetic linkage map SRAP SSR RAPD |
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