家蚕核型多角体病毒基因polh的诱变分析

曾报道经化学诱变剂ＭＭＣ、９－ＡＡ和ＥＭＳ诱变的家蚕核型多角体病毒（ＢｍＮＰＶ）多角体形态出现异常，继代分离的诱变ＢｍＮＰＶ基因组对ＥｃｏＲＩ、ＢｇｌＩＩ和ＢａｍＨＩ的酶切谱发生变化。研究进一步揭示，诱变ＢｍＮＰＶ的多角体外层蛋白晶格排列呈现紊乱；多角体蛋白的ＳＤＳ－ＰＡＧＥ电泳谱与对照组比较有显著差异；对多角体蛋白基因ｐｏｌｈ的测序结果显示，３组诱变ＢｍＮＰＶ的ｐｏｌｈ基因发生了多处碱基（氨基酸

Mutagenic Analysis on the Polyhedrin Gene (polh) of Bombyx mori Nuclear Polyhedrosis Virus (BmNPV)

In our early studies, the abnormal shape of the polyhedra of Bombyx mori nuclear polyhedrosis virus (BmNPV) induced by chemical mutagens of MMC. 9-AA and EMS occurred, and the genome of the mutated BmNPV obtained from the successive test had some change in the restriction endonuclease partners of EcoRI, BglII and BamH I. The present studies showed that the arrangement of the crystal lattice of the polyhedrin was disorderly, and the SDS-PAGE electropherogram of the polyhedrin depicted distinct change in comparison with control group. The results of sequencing analysis showed that many point mutations with characteristics of the base substitution had occured at some sites of the BmNPV polh gene in three mutated groups, and these results funther revealed molecular mutagenesis of the mutagens effective to BmNPV. It was not confirmable that the point mutations of polh gene in the mutated BmNPV have relationship to abnormal shape of the polyhedra.