| 通过农杆菌介导法将兔防御素NP-1基因导入毛白杨(P.tomentosa) |
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| 引用本文： | 赵世民,祖国诚,刘根齐,黄敏仁,徐金相,孙勇如.通过农杆菌介导法将兔防御素NP-1基因导入毛白杨(P.tomentosa)[J].遗传学报,1999(6). |
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| 作者姓名： | 赵世民 祖国诚 刘根齐 黄敏仁 徐金相 孙勇如 |
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| 作者单位： | 中国科学院遗传研究所!本?100101，南京林业大学!南京，210037，中国科学院遗传研究所!北京，100101，南京林业大学!南京，210037，中国科学院遗传研究所!北京，100101，中国科学院遗传研究所!北京，100101 |
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| 基金项目： | 国家自然科学基金!39770627 |
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| 摘 要： | 采用叶盘法将兔防御素NP－1基因导入毛白杨后，经PCR分析、Southern杂交检测与筛选获得了转基因植株。并经体外抑菌实验表明，转NP－1基因毛白杨植株组织提取物对某些微生物的生长具有明显的抑制作用。
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| 关 键 词： | 兔防御素基因 叶盘法 转化 毛白杨 |
Introduction of Rabbit Defensin NP-1 Gene into Poplar (P. tomentosa) by Agrobacterium-mediated Transformation |
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| ZHAO Shi-Min, ZU Guo-Cheng, LIU Gen-Qi, HUANG Min-RenXU Jin-Xiang, SUN Yong-Ru.Introduction of Rabbit Defensin NP-1 Gene into Poplar (P. tomentosa) by Agrobacterium-mediated Transformation[J].Journal of Genetics and Genomics,1999(6). |
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| Authors: | ZHAO Shi-Min ZU Guo-Cheng LIU Gen-Qi HUANG Min-RenXU Jin-Xiang SUN Yong-Ru |
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| Institution: | ZHAO Shi-Min; ZU Guo-Cheng; LIU Gen-Qi; HUANG Min-RenXU Jin-Xiang; SUN Yong-Ru(Institute of Genetics Chines Academy of Sciences Beijing 100101)(Nanjing Forest University Nanjing 210037) |
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| Abstract: | Rabbit defensin NP-1 possesses a broad resistant spectrum to pathogens. In this work, we have transferred the NP-1 gene into poplar plants by Agrobacterium-mediated transformation. PCR amplification and Southern analysis showed that rabbit defensin NP-1 gene was integrated into the poplar genome. The transformahon efficiency is about 15.6%. Antimicrobial achvity test showed that the extract of transgenic plants inhibited the growth of the tested microbes. |
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| Keywords: | Defensin gene Leaf discs Transgenic poplar |
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