| 提高籼稻愈伤组织再生频率的研究 |
| |
| 引用本文： | 田文忠.提高籼稻愈伤组织再生频率的研究[J].遗传学报,1994,21(3):215-221. |
| |
| 作者姓名： | 田文忠 |
| |
| 作者单位： | 中国科学院遗传研究所 |
| |
| 摘 要： | 为了提高籼稻愈伤组织的植株再生频率，研究了影响再生的各种因素，如：在诱导培养基或继代培养基中加细胞分裂素和萘乙酸（ＫＴ、ＢＡＰ、玉米素或Ｚｉｐ１毫克／升），或加Ｔｈｉｄｉａｚｕｒｏｎ（０．５毫克／升），以及愈及组织的部分干燥处理等。这些措施明显地提高籼稻愈伤组织的再生频率。结合使用这些处理可使ＴＮ１、ＩＲ７２和ＩＲ６４的愈伤组织再生植株频率较对照提高５－１４倍。
|
| 关 键 词： | 籼稻 组织培养 植株再生 |
Improvement Of Plant Regeneration Frequency in vitro In Indica Rice |
| |
| Tian Wenzhong.Improvement Of Plant Regeneration Frequency in vitro In Indica Rice[J].Journal of Genetics and Genomics,1994,21(3):215-221. |
| |
| Authors: | Tian Wenzhong |
| |
| Institution: | Tian Wenzhong(Institute of Genetics Academia Sinica Beijing 100101)Iann Rance;Elunialai Sivamani;Claude Fauquet;Roger N.Beachy(International Laboratory for Tropical Agricultural Biotechnology The Scripps Research Institute La Jolla CA 92037 USA) |
| |
| Abstract: | In order to improve the frequency of plant regeneration from calli in indica rice,the influences of different factors on plant regeneration were investigated.Supplement with cytokinins(KT,BAP,Zeatin or 2ip,mg/l)and NAA(1 mg/l),or supplement with thidiazuron(TDZ,5 mg/l)in the induction medium or subculture medium,and partial desiccation of callus before transfer to regeneration medium have been found significantly increase the frequency of plant regeneration in indica rice.5-14 folds more plants were obtained than untreated control by the combination of these treatments with indica varieties TN1,IR72 and IR64. |
| |
| Keywords: | Indica rice Tissue culture Regeneration plant |
| 本文献已被 CNKI 维普 等数据库收录！ |
