棉花纤维发生相关AFLP标记

以徐州142及其无绒无絮（无纤维）突变体(fl)为材料，利用AFLP标记技术对该近等基因系进行DNA多态性分析。在64对引物产生的6360多条扩增带中，发现1条差异带（CF1）稳定出现在有纤维和无纤维之间。进一步用徐州142无绒无絮突变系与纤维发育正常的渝棉一号的杂交后代F2、F3分离群体以及几个陆地棉品种作材料验证。结果表明，差异带（CF1）与纤维的产生呈共分离，该差异带是与棉花纤维发生相关的分子标记。进而对CF1进行回收、克隆和序列测定，CF1长205bp，通过6个开放阅读框推断的氨基酸序列，与酚羟化酶α-亚基、外表皮蛋白C、NADH脱氢酶亚基1、NADH－CoQ氧化还原酶、2－氧代－铁氧化蛋白－氧化还原酶以及假拟14．5kD蛋白（hypotheitcal 14.5kD protein）有较高的相似性。

An AFLP Marker Related to Fibrogenesis in Upland Cotton

By using AFLP technique polymorphism analysis was performed between a fuzzlesslintless mutant line and its isogenic wild-type line, Xuzhou 142. Out of 6 360 bands produced by 64 pairs primers, a fragment named as CF1, appearing stably in wild-type line, Xuzhou 142. This polymorphism was further verified using several normal fiber varieties and F2, F3 populations from the cross of fuzzless-lintless mutant line with a high-lint-percentage variety Yumian No. 1. The cosegregation of CF1 and fibrogenesis was proved, which suggested that CF1 can be used as a molecular marker for cotton fibrogenesis. The CF1 segment was cloned into PUCm-T Vector and then sequenced. The putative amino acid sequences, is an analogue to phenol hydroxylase alpha subunit outer surface protein C, NADH dehydrogenase subunit 1, NADH- ubiquinone oxidoreductase, 2-oxoacid ferredoxin oxidoreduct and hypothetical 14.5kD protein.