水稻叶绿素含量的QTL定位

利用由两个籼稻品种Acc8558和H359杂交构建的一个包含131个株系(F19)的重组自交系群体，及其相应的包含147个RFLP和78个AFLP标记的遗传图谱，采用多性状复合区间定位方法，对控制水稻叶绿素含量的QTL进行了定位分析。对叶绿素a和叶绿素b含量各检测到6个QTL，其中5个QTL在两性状问是相同的。这些QTL主要分布在第1和第4染色体上，因此这两条染色体对叶绿素含量是重要的。QTL qChlAlc／qChlBlb(二者位置相同)在4个观测时期均表现较大效应，且在最后的剑叶期贡献最大，因此对叶绿素含量最为重要。另两个QTL(qCh-LA4a／qChlB4a和qChlA4b／qChlB4b)只在第2次观测时期效应显著，表明它们只在特定发育阶段发挥作用。

Mapping of QTLs Controlling Chlorophyll Content in Rice

Mapping of QTLs controlling chrolophyll content was conducted with the method of multiple trait composite interval mapping,based on a recombinant inbred (RI) population consisting of 131 lines (F 10 ) derived from a cross between two indica rice varieties,Acc8558 and H359,and on a corresponding genetic map comprising 147 RFLP and 78 AFLP markers.Six QTLs for contents of chlorophyll a and chlorophyll b were detected,respectively.Among them,five QTLs were the same between the two characters.These QTLs were mainly distributed on chromosomes 1 and 4,indicating that the two chromosomes were more important for chlorophyll content. qChlA1c and qChlB1b (these two QTLs were mapped at the same location) showed relatively large effects at all the four observation times and showed the largest effects at the sword leaf period.Another two QTLs ( qChlA4a/qChlB4a and qChlA4b/qChlB4b ) exhibited significant effects only at the second observation time,suggesting that they might act only at specific developmental stages.