猪核糖体蛋白基因RPLP0的cDNA全长、染色体定位和多态性分析

RPLP0基因编码酸性核糖体磷蛋白大亚基P0, 是核糖体60S亚基的组成成分之一。从本室构建的猪胚胎骨骼肌cDNA文库中分离得到猪RPLP0基因的全长cDNA,并提交 GenBank 数据库。比较猪 RPLP0 基因和人及小鼠同源基因的cDNA序列和蛋白质序列,结果表明该基因在 3 个物种中具有高的相似性。用 PCR-RFLP 方法在猪 RPLP0基因cDNA 545处检测到 C→A 的单碱基突变,为 Csp6Ⅰ的酶切位点。统计分析结果表明 3 种基因型 (AA,AC,CC)在外来品种杜洛克,大约克, 长白和中国地方品种通城猪,小梅山,玉山猪中的分布各不相同。同时使用体细胞杂种板(SCHP)和辐射杂种板(IMpRH) 对 RPLP0 基因进行染色体定位,该基因被定位于 SSC 14q22-q24 并且和SW1321微卫星标志紧密连锁 (25cR, LOD = 14.54)。

Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLP0 Gene

RPLP0 gene encodes the acidic ribosomal phosphoprotein large PO subunit, which is a component of the 60S subunit.The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues. A C/A single nucleotide substitution in exon 5 was detected as Csp6 Ⅰ polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54).