普通小麦三种细胞质雄性不育系线粒体DNA的比较研究

对细胞质分别来源于粘果山羊草（Ａｅ．ｋｏｔｓｃｈｙｉ）、偏凸山羊草（Ａｅ．ｖｅｎｔｒｉｃｏｓａ）、提莫菲维（Ｔ．ｔｉｍｏｐｈｅｅｖｉ）的３种普通小麦雄性不育系，其相应保持系和共有的一种恢复系的ｍｔＤＮＡ进行了ＲＦＬＰ比较分析。发现Ｋ型和Ｖ型不育系的ｍｔＤＮＡ在组织结构上不同于Ｔ型，说明Ｋ、Ｖ型不育系是有别于Ｔ型的两种新不育类型。Ｋ型、Ｖ型不育系的ｍｔＤＮＡ与保持系和恢复系显著不同，推测ｍｔＤＮＡ与小麦细胞质雄性不育性有关。实验同时发现Ｔ型不育系与其保持系的ｍｔＤＮＡ非常相似，对这种相似性的原因进行了讨论。

The mtDNAs' RFLP Analysis of three Types of Cytoplasmic Male Sterility in Wheat

Three cytoplasmic male sterile lines(cms-K,V and T) in wheat,of which the cytoplasms detived from Ae. kotschyi,Ae. ventricosa and T. timopheevi respectively,were compared with their maintainers and a common restorer by RFLP analysis of mtDNA. The organization and structure of mtDNAs from cms-K and cms-V of wheat is different from that of cms-T,This means cms-K and cms-V are two new types of cytoplasmic male sterile lines.Distinct polymorphisms were identified among mtDNAs from cms-K, cms-V and their common maintainer,so mtDNA may involve in cms in wheat.The mtDNA of cms-T is very similar to its mamtainer's and the reason of the similarity was discussed. Received April 13,1994 1)Correspondence address:(Department of Agronomy.Shandong Agricultural University,Taiau 271018)