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甘蓝型黄籽油菜种皮色泽QTL作图
引用本文:刘列钊,孟金陵,林呐,谌利,唐章林,张学昆,李加纳.甘蓝型黄籽油菜种皮色泽QTL作图[J].遗传学报,2006,33(2):181-187.
作者姓名:刘列钊  孟金陵  林呐  谌利  唐章林  张学昆  李加纳
作者单位:1. 西南农业大学农学与生命科学学院,重庆,400716;华中农业大学作物遗传改良国家重点实验室,武汉,430070
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070
3. 西南农业大学农学与生命科学学院,重庆,400716
摘    要:甘蓝型黄籽油菜具有低纤维、高蛋白及高含油量的优点,因而己成为广大油菜育种工作者研究的重点之一。利用甘蓝型黑籽品系油研2号作父本,计蓝型黄籽品系GH06为母本,获得132个单株的F2群体;以AFLP和SSR为主要分析方法,构建了包括164个标记的甘蓝型油菜遗传连锁图谱,其中包括125个AFLP标记、37个SSR标记及一个RAPD和一个SCAR标记,分布在19个连锁群上,覆盖油菜基因组2549.8cM,标记间平均距离15.55cM。利用多区间作图法,对种皮色泽QTL进行分析,在第5及第19连锁群上各检测到一个QTL位点,分别解释表型变异46%及30.9%。

关 键 词:甘蓝型油菜  种皮色泽  简单重复序列  扩增片段长度多态性  连锁图谱  数量性状位点
收稿时间:2005-02-26
修稿时间:2005-02-262005-07-21

QTL Mapping of Seed Coat Color for Yellow Seeded Brassica napus
LIU Lie-Zhao,MENG Jin-Ling,LIN Na,CHEN Li,TANG Zhang-Lin,ZHANG Xue-Kun,LI Jia-Na.QTL Mapping of Seed Coat Color for Yellow Seeded Brassica napus[J].Journal of Genetics and Genomics,2006,33(2):181-187.
Authors:LIU Lie-Zhao  MENG Jin-Ling  LIN Na  CHEN Li  TANG Zhang-Lin  ZHANG Xue-Kun  LI Jia-Na
Abstract:The development of yellow-seeded varieties 9f Brassica napus for improving the oilseed quality characteristics of lower fiber content and higher protein and oil content has been a major focus of breeding researches worldwide in recent years. With the black-seeded 'Youyan 2' as male and the yellow-seeded GH06 as female parents respectively, F2 population of 132 individuals were obtained. A linkage map was constructed with 164 markers including 125 AFLP, 37 SSR, 1 RAPD and 1 SCAR markers distributed over 19 linkage groups covering approximately 2 549.8 cM with an average spacing of 15.55 cM. Two loci located on the 5th and 19th group were detected for the trait of seed coat color based on the linkage group using multiple interval mapping method and explained 46% and 30.9% of the phenotypic variation, respectively.
Keywords:Brassica napus  seed coat color  SSR  AFLP  linkage map  QTL
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