陆地棉体细胞胚胎发生与植株再生

利用陆地棉品种下胚轴为外植体进行体外培养研究。激素和品种是影响愈伤诱导和胚胎发生的主要因素。去除激素后胚性愈伤在固体培养基上只能形成少量的成熟胚。悬浮培养是获得大量成熟胚的中间步骤。悬培两周后,悬培物转到固体培养基上促进胚状体成熟,30—60目之间的悬培物比大于30目的悬培物易形成成熟胚。KT 0.1ppm、Zea 0.1ppm分别有效地促进了胚状体成熟。活性碳250mg/L、NAA 0.1ppm、IBA 0.1ppm和IAA 0.1ppm能使胚状体萌发并健壮生长。目前已得到100多株幼苗,大苗已达八片真叶。

Somatic Embryogenesis and Plant Regeneration in Upland Cotton

A great number of calli were obtained from seed hypocotyles of upland cotton (Gossyp-ium Hirsuium L.). The initiation of calli depended mainly on hormones. Calli were induced from all the varieties on the medium with 2,4-D, but the results were not very effective with NAA or IAA. Somatic embryogenesis of rhe variety Coker 201 initiated easily on the MS medium containing of Img/L IAA and Img/L KT. But the other seven varieties failed to produce embryoid. This suggested that there existed a relationship between embryogenesis and genotypes. Embryogenesis was most effectively induced on the medium with IAA. NAA was less effective, the poorest result was recorded on the medium with 2,4-D. It is probably that 2,4-D suppressed embryogenesis.Only a few mature embryoids had been obtained after the embryogenic cultures were transferred to the medium without hormones. Suspension culture was regarded as a intermediate step to promote embryoid maturity. Suspension cultures with 30-60 mesh sieve produced more matured and much bigger embryoids than 30 mesh sieve suspension cultures on the solid medium. KT 0.1mg/L. Zea 0.1mg/L were found to effective in promoting embryoid development. Active carbon, NAA O.lmg/L each stimulated germination and development of the matured embryoids. Over a hundred regenerated plants, some of which are now at eight-leaf srage, have been obtained.