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西藏白菜型油菜遗传多样性的RAPD分析
引用本文:王建林,旦巴,胡书银,涂金星,栾运芳,孟霞,卓嘎,尼玛卓玛,唐琳.西藏白菜型油菜遗传多样性的RAPD分析[J].遗传学报,2002,29(11):1021-1027.
作者姓名:王建林  旦巴  胡书银  涂金星  栾运芳  孟霞  卓嘎  尼玛卓玛  唐琳
作者单位:1. 西藏农牧学院农学系,林芝,860000
2. 华中农业大学农学系,武汉,430070
3. 西藏自治区农科所,拉萨,850002
基金项目:西藏油菜种质资源的生物多样性研究 (3 9960 0 41)
摘    要:通过利用22个10bp随机引物对来自西藏高原地区107份白菜型油菜种质资源材料的PAPD分析,探讨了西藏白菜型油菜品种之间的遗传分化关系,结果表明:(1)供试的107份材料共产生236条谱带,其中210条谱带有多态性,占88.98%,说明白菜型油菜在西藏高原地区具有较丰富的遗传多样性;(2)根据引物扩增出的DNA指纹图谱,运用UPGMA分析法,在遗传距离为0.078处,可将供试的107份白菜型油菜划分为11个类群,发现来自于同一地区或气候相似区的品种往往聚在一起,表明西藏高原白菜型油菜品种的相似性与其原产地的地理,气候背景密切相关,并在此基础上,结合西藏高原农业发展历史,气候背景以及地形地貌特点和植物地理学,植物区系学,植物进化论等方面的综合分析,提出西藏高原是世界白菜型油菜起源地的观点。

关 键 词:西藏  白菜型油菜  遗传多样性  RAPD分析
文章编号:0379-4172(2002)11-1021-07
修稿时间:2001年9月23日

RAPD Analysis for the Genetic Diversity of Brassica rapa in Tibet
WANG Jian Lin ,Dan Ba ,HU Shu Yin ,TU Jin Xing ,LUAN Yun Fang ,MENG Xia ,ZHUO Ga ,Nimazhuoma ,TANG Lin.RAPD Analysis for the Genetic Diversity of Brassica rapa in Tibet[J].Journal of Genetics and Genomics,2002,29(11):1021-1027.
Authors:WANG Jian Lin  Dan Ba  HU Shu Yin  TU Jin Xing  LUAN Yun Fang  MENG Xia  ZHUO Ga  Nimazhuoma  TANG Lin
Institution:WANG Jian Lin 1,Dan Ba 1,HU Shu Yin 1,TU Jin Xing 2,LUAN Yun Fang 1,MENG Xia 1,ZHUO Ga 1,Nimazhuoma 3,TANG Lin 3
Abstract:Tibet, a most beautiful place, locating in southwestern China. She has been called as the third pole of the earth. Unique geological history, complex land surface and climatic zones, various soil types, all different wild vegetations etc., all of these make Tibet a very typical area of vertical agricultural ecosystem. The ecosystem in Tibet may be the most complex in the world, which varies from place to place. Genetic differentiation of 107 accessions of Brassica rapa from Tibet plateau was studied by DNA PAPD analysis using 2210bp random primers, the genetical distribution in 107 accession of Brassica rapa from Tibet plateau was found. The results are as follows: (1) Total 236 bands were produced from 107 Tibet oilseed accession of B. rapa germaplasm resource in Tibet, of 210 bands amplified from B. rapa germaplasm resource showed polymorphism, with the ratio 88.98%. The result showed that oilseed accession of B. rapa in Tibet has richer genetic diversity; (2) Dendrogram constructed from DNA RAPDs showed that 107 accessions of B. rapa from Tibet plateau were devided into 11 cluster by calculating genetic distance, the cluster analysis showed that the genetic variation among oilseed accessions of B. rapa was closely related with their eco geographic distribution; extensive variation existed among the accessions from Tibet Province. Based on the analysis of unique geological history, complex land surface and climatic zones, various soil types, complex growing environments, longagricultural history, different cropping systems, and natural and artificial selection as well as plant geography, plant evolution theory, it concludes that Tibet is one of the oil seed gene centers in the word.
Keywords:tibet  oilseed  Brassia rapa  genetic diversity  RAPD
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