| 建立小鼠胚胎多能干细胞系（ES细胞系）的几个主要影响因素 |
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| 引用本文： | 尚克刚,李子玉.建立小鼠胚胎多能干细胞系（ES细胞系）的几个主要影响因素[J].遗传学报,1992,19(6):491-496. |
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| 作者姓名： | 尚克刚 李子玉 |
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| 作者单位： | 北京大学生物学系 100871
(尚克刚，李子玉)，北京大学生物学系 100871(吴鹤龄) |
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| 摘 要： | 本文系统地比较了影响建立小鼠胚胎多能干细胞系的几个主要因素。对825个胚的实验进行了分析,其结果表明:延迟囊胚的主要作用是有助于内细胞团(ICM)的增殖;高糖的DMEM有助于囊胚贴壁及ICM的增殖;不含丙酮酸钠的DMEM对ES细胞的集落形成和生长是有利的;饲养层对ES细胞的建系和培养是十分重要的,以新鲜制备的原代小鼠胚胎成纤维细胞作为饲养层对ES细胞的生长有明显的促进作用。
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| 关 键 词： | 小鼠 影响因素 多能干细胞系 |
Effects of Several Primary Factors Related to Establishment of Mouse Embryo Pluripotent Stem Cell Lines (ES Cell Line) |
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| Shang Kegang Li Ziyu Wu Heling.Effects of Several Primary Factors Related to Establishment of Mouse Embryo Pluripotent Stem Cell Lines (ES Cell Line)[J].Journal of Genetics and Genomics,1992,19(6):491-496. |
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| Authors: | Shang Kegang Li Ziyu Wu Heling |
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| Abstract: | Several primary factors related to establishment of mouse embryo pluripotent stem cell lines (ES cell lines) have been tested compaiatively. The results of 825 embryos indicated: (1) Delayed blastocysts were more conducive than normal ones to proliferation of inner cell mass (ICM). (2) Culture medium DMEM with 4 500mg/l glucose was more advantageous than DMEM with 1000mg/l glucose to the attachment of explanted blastocysts and to the the proliferation of ICM. (3) DMEM without sodium pyruvate was beneficial to appearance of ES ceil colonies and to their growth. (4) Feeder layer prepared from the primary culture of mouse embryos had very good effects to the growth and culture of ES cells. |
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| Keywords: | Mouse ES cell Establishment line Effected factors |
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