| A cotton mitogen-activated protein kinase （GhMPK6） is involved in ABA-induced CAT1 expression and H2O2 production |
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| 作者姓名： | Luo J Zhao LL Gong SY Sun X Li P Qin LX Zhou Y Xu WL Li XB |
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| 作者单位： | Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China |
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| 基金项目： | This work was supported by the project from Ministry of Agriculture of China for transgenic research （Nos. 2009ZX08009-117B and 2011ZX08009-003） and Ministry of Education of China （Nos. 20070511001 and 200805111023）. |
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| 摘 要： |
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| 关 键 词： | 丝裂原活化蛋白激酶 ABA诱导 过氧化氢 生产 棉花 转基因植物 MAPK 信号通路 |
A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H(2)O(2) production |
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| Luo J,Zhao LL,Gong SY,Sun X,Li P,Qin LX,Zhou Y,Xu WL,Li XB.A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H(2)O(2) production[J].Journal of Genetics and Genomics,2011,38(11):557-565. |
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| Authors: | Luo Juan Zhao Li-Li Gong Si-Ying Sun Xiang Li Peng Qin Li-Xia Zhou Ying Xu Wen-Liang Li Xue-Bao |
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| Institution: | Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China |
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| Abstract: | The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays a pivotal role in the regulation of stress and developmental signals in plants.Here,we identified one gene,GhMPK6,encoding an MAPK protein in cotton.GFP fluorescence assay demonstrated that GhMAPK6 is a cytoplasm localized protein.Quantitative RT-PCR analysis revealed that mRNA accumulation of GhMPK6 was significantly promoted by abscisic acid (ABA).Overexpression of GhMPK6 gene in the T-DNA insertion mutant atmkkl (SALK_015914) conferred a wild-type phenotype to the transgenic plants in response to ABA.Under ABA treatment,cotyledon greening/expansion in GhMPK6 transgenic lines and wild type was significantly inhibited,whereas the atmkkl mutant showed a relatively high cotyledon greening/expansion ratio.Furthermore,CAT1 expression and H2O2 levels in leaves of GhMPK6 transgenic lines and wild type were remarkably higher than those of atmkkl mutant with ABA treatment.Collectively,our results suggested that GhMPK6 may play an important role in ABA-induced CAT1 expression and H2O2 production. |
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| Keywords: | Cotton (Gossypium hirsutum) Mitogen-activated protein kinase (MAPK) Hydrogen peroxide (H2O2) Abscisic acid (ABA) Hypersensitivity |
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