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| 水稻抗褐飞虱基因bph2的SSR定位和标记辅助选择 | |
| 引用本文： | 孙立宏,王春明,苏昌潮,刘裕强,翟虎渠,万建民.水稻抗褐飞虱基因bph2的SSR定位和标记辅助选择[J].遗传学报,2006,33(8):717-723. |
| 作者姓名： | 孙立宏 王春明 苏昌潮 刘裕强 翟虎渠 万建民 |
| 作者单位： | 1. 南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程研究中心,南京,210095 2. 中国农业科学院作物科学研究所,北京,100081 3. 南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程研究中心,南京,210095;中国农业科学院作物科学研究所,北京,100081 |
| 基金项目： | 国家高技术研究发展计划(863计划);教育部资助项目;引进国际先进农业科技计划(948计划) |
| 摘 要： | 利用综合性状较好对褐飞虱敏感的粳稻恢复系C418为父本，以含有bph2基因的抗褐飞虱品种ASD7为母本构建了包含134个F23家系的群体，利用苗期鉴定法对F2：3家系进行抗性鉴定：用SSR标记技术，将bph2基因定位在第12染色体长臂上，标记RM7102和RM463之间，其遗传距离分别为7．6cM和7．2cM。在进行表型选择的同时，利用与bph2基因连锁的SSR标记RM7102和RM463对BC1F1和BC2F1进行了标记辅助选择，选择效率分别为89．9％和91．2％，为培育高抗褐飞虱水稻品种奠定了基础。 |
| 关 键 词： | 水稻 褐飞虱 抗虫性 分子标记辅助选择 |
| 收稿时间： | 2005-06-07 |
| 修稿时间： | 2005-06-072005-08-09 |
Mapping and Marker-assisted Selection of a Brown Planthopper Resistance Gene bph2 in Rice (Oryza sativa L.) |
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| SUN Li-Hong,WANG Chun-Ming,SU Chang-Chao,LIU Yu-Qiang,ZHAI Hu-Qu,WAN Jian-Min.Mapping and Marker-assisted Selection of a Brown Planthopper Resistance Gene bph2 in Rice (Oryza sativa L.)[J].Journal of Genetics and Genomics,2006,33(8):717-723. | |
| Authors: | SUN Li-Hong WANG Chun-Ming SU Chang-Chao LIU Yu-Qiang ZHAI Hu-Qu WAN Jian-Min |
| Institution: | 1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095 China; 2. Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | Nilaparvata lugens Stal (brown planthopper, BPH), is one of the major insect pests of rice (Oryza sativa L.) in the temperate rice-growing region. In this study, ASD7 harboring a BPH resistance gene bph2 was crossed to a susceptible cultivar "C418". SSR assay and linkage analysis were carried out to detect bph2. As a result, the resistant gene bph2 in ASD7 was successfully mapped between RM7102 and RM463 on the long arm of chromosome 12, with distances of 7.6 cM and 7.2 cM,respectively. Meanwhile, both phenotypic selection and marker-assisted selection (MAS) were conducted in the BC1F1 and BC2F1populations. Selection efficiencies of RM7102 and RM463 were determined to be 89.9% and 91.2%, respectively. It would be very beneficial for BPH resistance improvement by using MAS of this gene. |
| Keywords: | SSR Oryza sativa L Nilaparvata Lugens Stal insect resistance SSR marker-assisted selection |
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