五龄家蚕若干组织器官蛋白质数据库构建

为构建家蚕蛋白质数据库，达到从蛋白质水平研究基因的表达及表达产物的加工修饰的目的，采用五龄家蚕休体壁、中肠和脂肪为材料获取蛋白质样品，用蛋白质双向电泳技术分离、纯化蛋白质，对其中的40个蛋白质斑点进行了氨基酸序列分析及同源性检索，发现其中58.5％的蛋白质与果蝇的有关蛋白质具有较高同源性，36.5％与其他生物的蛋白质具有较高同源性，只有5％与家蚕已知蛋白拷贝产高同源性，研究发现有27个蛋白质的N－末端氨基酸序列在家蚕中是第一次被检测到，并通过互联网向SWISS－PROT数据库进行了登录。研究证明利用蛋白质数据库的结果，可以得到基因表达及其产物修饰的信息。

Protein Databank for Several Tissues Derived from Five Instar of Silkworm

We attempted to construct protein databank of silkworm (Bombyxmori L.) for clarifying the gene expression and post-translational modification. Proteins from silkworm body wall, fat body and middle intestines were separated by two-dimensionalelectrophoresis. The N-terminal amino acid sequences of 40 proteins and their homology to proteins from other organisms were determined. 58.5% proteins were high homologous to the already reported proteins in D. Melanogaster and 36.5% to those in other organisms. Only 5% of the proteins were homologous to the known protein in silkworm. The N-terminal sequences of 27 proteins were first found in silkworm, and all these data were registered in SWISS-PROT through the Internet. The results suggested that the information of gene expression and post-translational modification could be obtained by the information of protein databank.