睾丸生精细胞凋亡相关基因TSARG1与Mtsarg1的分子克隆及Mtsarg1基因的表达谱分析

从已获得的在隐睾和正常睾丸对照中表达量有明显差异的EST片段（GenBank登录号：BE644538）出发，利用生物信息学和实验技术，克隆了小鼠睾丸生精细胞凋亡相关新基因Mtsarg1及相应的人类新基因TSARG1，Gen-Bank登录号分别为AF399971和AY032925。小鼠Mtsargl与人类TSARGl基因在氨基酸水平有55％的一致性和61％相似性，与其他已知蛋白质无明显同源性。小鼠10种组织的RT-PCR分析结果表明，Mtsargl基因在睾丸中高表达，在附睾中呈微弱表达，在其他组织不表达，提示Mtsargl和TSARGl基因在生精细胞凋亡或精子发生中具有潜在的重要作用。

Molecular Cloning for Testis Spermatogenesis Cell Apoptosis Related Gene TSARG1 and Mtsarg1 and Expression Analysis for Mtsarg1 Gene

Spermatogenesis cell apoptosis is a very complex process,which needs many molecules to take part in the programmable death of cells in testis.At present,research of apoptosis for spermatogenesis cell is at the primary step.It is very important to clone spermatogenesis cell apoptosis related genes and spermatogenesis genes in testis.Applying the bioinformatics and experiment technique,we have cloned human and mouse novel gene cDNA sequences-human testis and spermatogenesis cell apoptosis related gene 1 ( TSARG1 ) and mouse testis and spermatogenesis cell apoptosis related gene 1( Mtsarg1 ) from human and mouse testis cDNA library respectively, using a cDNA fragment (GenBank accession number:BE644538) as an electronic probe,which was significantly changed in expression in cryptorchidism.The GeneBank accession numbers of Mtsarg1 and TSARG1 are AF399971 and AY032925 (NM139073), respectively.The Mtsarg1 has a 55% identity and 61% similarity with TSARG1 at the amino acid level,which did not share significant homology with any other known protein in databases.The full-length cDNA of TSARG1 gene is 973 bp,including 549 bp open reading frame(ORF) and coding 183 amino acids,whereas the full-length cDNA of Mtsarg1 gene is 1103 bp,including 576 bp ORF and coding 192 amino acids.The predicted molecule weight of TSARG1 is 19948 61 Dolton,and the deduced iso-electric point is 10 24,whereas the Mtsarg1 is 20875 93 and is 9 83,being alkaline proteins.RT-PCR analysis showed that Mtsarg1 was expressed significantly in testis and faintly in epididymis in the ten tissues of testis,ovary,spleen,kidney,lung,heart,brain,epididymis,liver and skeletal muscle in mouse,while it wasn't expressed in the other eight tissues.Therefore,our results suggested that Mtsarg1 and TSARG1 would be pay potential roles in spermatogenesis cell apoptosis or spermatogenesis.