用ＰＣＲ—ＳＳＯ方法研究云南西部彝族ＨＬＡ—ＤＱＢ１基因的多态性

应用ＰＣＲ－ＳＳＯ基因分型技术，对我国云南西部地区３代内无血缘关系的７６个彝族健康个体进行了ＬＡ－ＤＱＢ１位点的基因分型。结果显示，在ＤＱＢ１的３８个等位基因中，观察到１３个等位基因，云南西彝族表现为ＤＱＢ１＊０３０１（３６．１８％－３６．８４％）最常见。其他频率大于５％的等位基因还有ＤＱＢ１＊０５０２（１０．５３％－１１．１８％）、ＤＱＢ１＊０４０１（９．２１％）、ＤＱＢ１＊０３０２（８．５５％－９．２１％）、ＤＱＢ１*0601(7．８９％）ＤＱＢ１＊０５０３１（６．５８％）、ＤＱＢ１＊０３０３２（５．９２％－６．５８％）。和其他１３个华人群体ＤＱＢ１等位基因的频率比较分析表明，总体上，云南西彝族和其他各华人群体间都存在很大的差异。显示其ＨＬＡ等位基因频率分布的民族独特性。

Analysis of HLA-DQB1 Polymorphism by PCR-SSO in Yichu of Yunnan Province

HLA-DQB1 genes from 76 individuals of Yichu ethnic group in Yunnan Province were investigated, using PCR-SSO genotyping method. Of the 38 DQB1 alleles detected, DQB10301 (gene frequency: 36.18%～36.84%) was the most common gene. The frequencies of DQB10502(10.53%～11.18%), DQB10401 (9.21%), DQB10302(8.55%～9.21%), DQB10601(7.89%), DQB105031(6.58%), and DQB103032(5.92%～6.58%) are more than 5%. While DQB10504, DQB10604, DQB106052, DQB10606, DQB10607, DQB10608, DQB106112, DQB10613, DQB10615, DQB10203, DQB10305, DQB10306, DQB10307, and DQB10308 were not observed. Comparison of HLA-DQB1 allele frequencies of Yichu with those of 13 other Chinese ethnic groups showed some significant differences, suggesting Yichu is unique in the distribution of HLA alleles.