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新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析
引用本文:林忠旭,张献龙,聂以春.新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析[J].遗传学报,2004,31(6):622-626.
作者姓名:林忠旭  张献龙  聂以春
作者单位:华中农业大学作物遗传改良国家重点实验室,武汉,430070
基金项目:国家“8 63”高技术项目 (编号 :2 0 0 1AA2 1112 1、2 0 0 2AA2 110 3 1),国家“948”引进项目 (编号 :2 0 10 12 )资助~~
摘    要:将新型分子标记SRAP(Sequence-related Amplified Polymorphism)应用于棉花的遗传研究,并建立了完整的PCR反应体系,此体系稳定可靠、扩增效果好、可重复性强。采用30个SRAP引物组合对海岛棉品种“Pima90”和陆地棉品种“邯郸208”进行比较扩增,29个引物组合可以获得多态性扩增,显示了较高的多态性。对上述两个品种的F2群体进行检测,共产生149个多态性条带,平均每个组合产生5.14个,单引物组合最多可产生13个多态性条带。用SRAP标记对11份陆地棉材料进行遗传多样性检测,30个引物组合中15个组合有多态性,得到22个多态性条带,显示了较高的多态性比率。研究结果表明,SRAP标记可在棉花分子生物学领域中广泛应用。

关 键 词:棉花  SRAP  遗传多样性
文章编号:0379-4172(2004)06-0622-05

Evaluation of Application of a New Molecular Marker SRAP on Analysis of F2 Segregation Population and Genetic Diversity in Cotton
Abstract:SRAP(Sequence-related Amplified Polymorphism),a new marker system,was applied in cotton genome analysis.We developed an efficient PCR reaction system for detecting SRAP that showed reliable,effective and reproductive.SRAP marker was used to detect the polymorphisms between 'Pima90'(G.barbodense) and 'Handan208'(G.hirsutum) and showed comparatively high polymorphism.Among the 30 primer pairs,29 pairs generated 149 polymorphic bands with an average of 5.14 polymorphic bands per primer pair.The primer pair showing most polymorphism was the combination of me3 and em2,which produced 13 polymorphic bands.The SRAP marker was also tested among 11 upland cotton cultivars,15 of the tested 30 primer pairs showed polymorphism yielding 22 polymorphic genetic loci,which is relatively high among upland cotton cultivars.The results showed that SRAP marker could be widely used in molecular genetic map construction and germplasm evaluation of cotton.
Keywords:cotton  SRAP(sequence-related amplified polymorphism)  genetic diversity
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