| 转OMT／PGH基因猪外源基因整合及遗传特性研究 |
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| 引用本文： | 樊俊华,陈清轩.转OMT／PGH基因猪外源基因整合及遗传特性研究[J].遗传学报,1999,26(5):497-500. |
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| 作者姓名： | 樊俊华 陈清轩 |
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| 作者单位： | [1]湖北省农业科学院畜牧兽医研究所 [2]中国科学院发育生物学研究所 |
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| 基金项目： | 国家2863”计划委员会资助 |
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| 摘 要： | 实验以转ＯＭＴ／ＰＧＨ基因猪的Ｇ０，Ｇ１，Ｇ２和Ｇ３代共８头猪为材料，应用同位素和非同位素标记的染色体原位杂交技术，对外源ＯＭＴ／ＰＧＨ基因在猪染色体上整合位点进行研究，结果表明：（１）外源基因可以整合在染色体上，整合的位点是随机的。（２）整合在染色体上的外源基因可以遗传给子代；（３）整合在染色体上的外源基因在转基因动物世代过程中整合的位点是相对稳定的。
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| 关 键 词： | 原位杂交 染色体 整合位点 转基因猪 |
A Study of Genetic Character and Foreign OMT/ PGH Gene Integration in Transgenic Swine |
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| FAN Jun-Hua, WEI Qing-Xin, CHEN Qing-Xian, LI Kui.A Study of Genetic Character and Foreign OMT/ PGH Gene Integration in Transgenic Swine[J].Journal of Genetics and Genomics,1999,26(5):497-500. |
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| Authors: | FAN Jun-Hua WEI Qing-Xin CHEN Qing-Xian LI Kui |
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| Institution: | Institute of Animal and Veterinary HAAS Wuhan 430064 |
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| Abstract: | By using isotopc and non-isotopic in situ hybridization on chromosomes, thelocus on chromosomes of foreign OMT/ PGH gene was analyzed in eight transgenicpigs (G0, G1 G2 and G3). The research result is as follows:(1) The foreign genes couldintegrated on chromosomes of pig and the foreign integrated randomly, (2) the foreigngenes integrated on chromosomes were bosndssible through generahons; (3) the locuson chromosomes of foreign gene was relatively stable in altemation of generations intransgenic swine. |
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| Keywords: | in situ hybridization Chromosomes Integrated Transgenic swine |
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