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小麦抗白粉病基因Pm21的分子鉴定和标记辅助选择
引用本文:刘志勇,李洪杰.小麦抗白粉病基因Pm21的分子鉴定和标记辅助选择[J].遗传学报,1999,26(6):673-682.
作者姓名:刘志勇  李洪杰
作者单位:[1]中国农业大学植物遗传育种系 [2]河北省农林科学院粮油作物研究所
基金项目:国家自然科学基金!39670508
摘    要:利用小麦抗白粉病基因Pm21的RAPD标记、SCAR标记和荧光源位杂交技术对小麦抗病育种材料中的抗白粉病Pm21基因进行了分子鉴定和标记辅助选择。

关 键 词:Pm21  RAPD  SCAR  小麦抗病育种  FISH  DNA分子标记

Molecular Identification and Marker-assisted Selection of Pm21 Gene Conferring Resistance to Powdery Mildew in Wheat
LIU Zhi-Yong, SUN Qi-Xin, LI Hong-Jie, NI Zhong-Fu, YANG Zuo-Min,TANG Bo-Rang, YANG Ai-Deng, JIA Xu.Molecular Identification and Marker-assisted Selection of Pm21 Gene Conferring Resistance to Powdery Mildew in Wheat[J].Journal of Genetics and Genomics,1999,26(6):673-682.
Authors:LIU Zhi-Yong  SUN Qi-Xin  LI Hong-Jie  NI Zhong-Fu  YANG Zuo-Min  TANG Bo-Rang  YANG Ai-Deng  JIA Xu
Abstract:RAPD marker (OPH17(1400)) and SCARmarkers (SCAR1400 and SCAR(1265)) as well as fluorescence in situ hybridization (FISH) technique were used to detect wheat powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/ 6VS) in different genetic background of wheat breeding program. Among a total of 372 RAPD amplification reactions, 28 reastions (7.53%) yield no ampliflcation products, and the amplification products of 21 reactions (5.64%) were difficult to interpret the presence or absence of the OPH17(1400), indicating that RAPD marker OPH17(1400) is less reproducible and reliable, so that its usage in breeding program is limited. However, SCAR markers, SCAR(1400) and SCAR(1265), were amplified in all 488 PCR reactions, suggesting that SCAR markers are highly reproducible and reliable, and can be used in breeding Program. The specific SCAR(1265) made it possible to screen a large number of genotypes reliably and rapidly in practical breeding programs for the presence/absence detection between the resistant and susceptible plants. After backcrossed with common wheat lines for several bines, no recombination between the 6VS chromosome of Haynaldia villosa and any of the wheat chromosomes was detected by using FISH.
Keywords:Wheat resistance breeding  Powdery mildew  Pm21  RAPD  SCAR  FISH  Marker-assisted selection
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