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中国西北地区汉,回,维,藏民族HLA—DRB基因多态性的研究
引用本文:赖淑苹,任惠民.中国西北地区汉,回,维,藏民族HLA—DRB基因多态性的研究[J].遗传学报,1999,26(5):447-457.
作者姓名:赖淑苹  任惠民
基金项目:国家自然科学基金!3940071
摘    要:按照第11届国际相容性抗原研讨会工作会议HLAⅡ类PCR-SSO分型标准和美国国立骨髓供者计划组织对HLA DRB位点等位基因分型要求,设计合成1对引物,扩增HLA DRB DNA片段,长度为256bp,设计合成不同片段大小探针27种,可检出DRB座位上DRB1的39种等位基因,DRB3的3种等位基因,DRB4的1种等位基因和DRB5的34种等位基因。

关 键 词:HLA-DRB位点  西北地区  等位基因  多态性  民族

HLA-DRB Alleles Polymorphism in Han, Hui, Uygur and Tibetan Populations in Northwestern China
LAI Shu-Ping REN Hui-Min HU Hai-Tao LI Sheng-Bin LAI Jiang-Hua,YAN Chun-Xia ZHANG Hong-Bo ZHAO Jun-Hai.HLA-DRB Alleles Polymorphism in Han, Hui, Uygur and Tibetan Populations in Northwestern China[J].Journal of Genetics and Genomics,1999,26(5):447-457.
Authors:LAI Shu-Ping REN Hui-Min HU Hai-Tao LI Sheng-Bin LAI Jiang-Hua  YAN Chun-Xia ZHANG Hong-Bo ZHAO Jun-Hai
Abstract:According to the llth THW standard, we designed a pair of primers by which asegment of 256bp of HLADRB1, B3, B4, and B5 could be amplified simultaneously.Twenty-seven oligonucleohde probes were designed and synthesized for 39 loci onDRB1, 3 on DRB3, 1 on DRB4 and 3 on DRB. A PCRSSO DNA typing protocolwas built which met the standard of HLA class II DNA typing in llth IHW. Withthes method, 186 of Chinese (Han) in Xi'an, 169 of Hu in Mngxia, 200 of Uygurin Xinjiang, and 188 of Tibetan in Tibet were detected with a comparison study. Thegenetic distribution of 46 loci of DRB in four ethnic healthy populations weresurveyed. No diversity has been found between Han and Hin in DRB DRBl 02(16.9%), DRBl * 07 (13.1%), and DRBl * 09(12.0%) were the higher frequent lociin Han. More DRB loci were detected in Tibetan but without higher loci as in Hanand Hui. The DRB polymorphism of Uygur population was similar to Caucasian. Adiscrindnahve highest frequency of DRBl * 07(23.8%) was found in Uygur, whichimpied a selechon happened.
Keywords:HLA-DRB loci  PCR-SSOP  Allelic polymorphism  Han  Hui  Uygur  Tibetan
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