太湖猪遗传多样性和系统发生关系的RAPD分析

报道了对太湖猪７个类群，红灯笼猪，约克夏种猪及太湖地区野猪共５７个个体随机扩增多态ＤＮＡ（ＲＡＰＤ）分析的结果，重建了太湖猪的系统发生树。实验从４０个引物中选用重复性较好的１３个引物对所有个体进行了随机扩增，共获得１９８个ＲＡＰＤ标记，单个引物获得的标记数在３－１６间。

RAPD Analysis of Genetic Diversity and Phylogenetic Relationship of,the Taihu Pigs

The genetic diversity and phylogenetic relationship of the Taihu pigs were studiedusing RAPD technique. A total of 57 individuals were used, and the phylogenetic treewas reconstructed using UPGMA method based on 198 RAPD markers which wereamplified with 13 decamer primers. The results indicate as the following f (1) Thesimilarity indexes within each strain and between the strains of the Taihu pigs are allhigh. The average genetic distance between the strains is 0.052. These results indicatethat the Taihu pigs exhibit relahvely low level of genehc variation and poor geneticdiversity; (2) The Hongdenglong pig is also a strain of the Taihu pigs, (3) The Taihupigs have close phylogenehc relahonship with the Chinese wild boar occured in theTaihu Lake area; (4) Two lineages diverged in the eary stage during the course ofthe formahon of the Taihu pigs. One of the lineage gave rise to the strains of Mizhu,Erhualian, Jiaxinhei and Hongdenglong, another lineage gave rise to the strains ofShawutou, Meishan, Fengiing and Hengjing. The strategy for genehc diversityconservation of the Taihu pigs was discussed.