首页 | 本学科首页   官方微博 | 高级检索  
   检索      

RAPD技术在玉米自交系亲缘关系研究中的应用
引用本文:孙致良,张超良.RAPD技术在玉米自交系亲缘关系研究中的应用[J].遗传学报,1999,26(1):61-68.
作者姓名:孙致良  张超良
作者单位:[1]莱阳农学院 [2]中国科学院遗传研究所
基金项目:国家九五科技攻关项目!96-014-01-06(3),山东省农业厅重点科研项目!9562203C
摘    要:通过对我国正在使用的12个玉米骨干自交系的RAPD分析,从220个Operon引物中筛选出12个能产生稳定的遗传多态性的引物,利用这些引物扩增出的指纹图谱,进行聚类分析,可将全部供试自交系分成3个类群,第1类群包括黄早4系统的5个自交系;第2类群包括478和488两个姊妹系;第3类群包括5个关系较远的自交系,其中3个来自美国,1个是全部中国血统,1个既有美国血统又有中国血统。这个结果与根据各个自交

关 键 词:玉米  自交系  RAPD标记  聚类分析

Application of RAPD Analysis in the Study of Phylogenetic Relationship among Maize Inbred Lines
SUN Zhi-Liang, ZHANG Chao-Liang, JIN De-Min, SUN Shi-Mong, GUO Bao-Tai, SUN Ke-Lian, WANG Bin.Application of RAPD Analysis in the Study of Phylogenetic Relationship among Maize Inbred Lines[J].Journal of Genetics and Genomics,1999,26(1):61-68.
Authors:SUN Zhi-Liang  ZHANG Chao-Liang  JIN De-Min  SUN Shi-Mong  GUO Bao-Tai  SUN Ke-Lian  WANG Bin
Institution:SUN Zhi-Liang; ZHANG Chao-Liang; JIN De-Min; SUN Shi-Mong; GUO Bao-Tai; SUN Ke-Lian; WANG Bin ( Institute of Genetics Chinese Academy of Sciences Beijing 100101) ( Laiyang Agricultural College Laiyang 265200)
Abstract:Twelve maize inbred lines were examined for RAPD analysis with 220 ten-mer Opron primers. Of them, 12 were selected for cluster analysis because they gave reproducible, polymorphic fingerprintings. Dendrogram constructed from RAPD data showed that twelve maize inbred lines were devided into three clusters. The first cluster consists of Huangzao-4 and four inbred lines whose one parent is Huangzao-4; 502-196 and 502 form the first subgroup since 502-196 is an improved inbred line of 502; Huangzao-4 and Wenhuang-31413 form the second subgroup; H21 forms the third subgroup because its another parent is an American inbred line. The second cluster includes two inbred lines, 478 and 488, which came from a same F2 plat. The five remaining inbred lines form a separate cluster for their different origin; Mol7, a well-known American inbred line, forms the first subgroup; 107 and 81 12, which also came from the United States, form the second subgroup; 340 and 515 form the third subgroup, 340 is a native inbred line whose parents are Chinese local varieties and 515 has American and Chinese genealogy. These results indicate that phylogenetic relationship determined by RAPD analysis is basically consistent with the pedigree of the twelve maize inbred lines.
Keywords:Maize inbred lines  RAPD markers  Cluster analysis
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号