人Elongator复合物Elp4亚基基因可部分补偿酵母ELP4缺失菌株的生长缺陷

为研究人Elongator复合物EIp4亚基的功能，将人ELP4基因转入酵母ELP4基因缺失的突变菌株(elp4△菌株)中，并对转化菌株进行功能互补实验和SSA3和PHO5基因表达分析，结果表明人的ELP4基因不能恢复突变菌株对高盐的敏感性，但可以在一定程度上缓解突变菌株对高温、咖啡因(Caffeine)和6-氮尿嘧啶(6-AU)的敏感性，部分恢复低磷条件下PHO5基因表达延迟的缺陷，并可在热激条件下提高SSA3基因的表达，因此人的ELP4基因可以部分补偿酵母ELP4基因缺失所引起的生长缺陷，提示人的EIp4亚基可能与酵母的该亚基功能相似。

The Elp4 Subunit of Human Elongator Complex Partially Complements the Growth Defects of Yeast ELP4 Deletion Strain

In this study,we performed in vivo experiments to determine the function of human Elongator subunit Elp4 by using a yeast complementary system.Our results indicated that though human ELP4 was not able to complement the growth defects of the ELP4 deletion mutant strain to high concentration salt,it partially reduced the sensitivity of mutant strain to caffeine,high temperature and 6-AU.Gene expression analysis indicated that human ELP4 partially resumed the slow activation of the PHO5 gene caused by the deletion of yELP4 under the low phosphate concentration.Meanwhile,under the condition of heat shock treatment,hELP4 increased the expression level of SSA3 gene.All these data demonstrated that human ELP4 can partially complement the growth defects and restore the slow activation of certain genes of the yELP4 deletion strain.These results indicate that human Elp4 subunit has similar functions to that of the yeast.