用RAPD标记评估我国棉花品种遗传多样性

利用ＲＡＰＤ标记对１７个不同来源的我国棉花品种（系）进行遗传多样性评估。２００个随机引物扩增出１１３条多态性片段。１７个品种（系）依据染色体组的不同可以分为３大类。它们之间遗传相似性系数从０．２０００到０．８４５１。３个大面积推广品种鄂荆１号，中棉所１２号和苏棉３号遗传基础十分相近。陆地棉与阔叶棉杂交产生的野生种质系７５８１，与其他陆地棉品种（系）遗传差异显著。不同育种单位在育种过程中种质使用具有

Genetic Diversity Evaluation of Some Chinese Elite Cotton Varieties with RAPD Markers

RAPDs analysis was used to assess the genetic diversity of some elite cotton varieties. 17 cotton varieties or breeding lines were screened with 200 arbitrary primers to generate 113 polymorphic bands. Cluster analysis and UPGMA showed that 17 varieties could be put into 3 clusters corresponding to genome difference. The genetic similarity among them ranged from 0.2000 to 0.8451. Three elite varieties Ejing-1, Sumian-3 and Zhongmiansuo-12 are highly similar in genetic base. 7581, an offspring from Gossypium hirsutum. hirsutum race Latifolium, is distinct from other upland cotton varieties. A trend in germplasm usage in the same research center was also shown in the study. To sustain progress in genetic improvement, we suggest that some good gene from other species should be considered in the upland cotton breeding procedure.