| 用SSR标记研究柑橘属及其近缘属植物的亲缘关系 |
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| 引用本文： | 庞晓明,胡春根,邓秀新.用SSR标记研究柑橘属及其近缘属植物的亲缘关系[J].遗传学报,2003,30(1):81-87. |
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| 作者姓名： | 庞晓明 胡春根 邓秀新 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,国家农作物分子育种中心,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助 (No .39970 0 81)~~ |
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| 摘 要： | 用SSR标记分析了29份柑橘属及近缘属植物的亲缘关系。7对SSR引物在29个样品中扩增得到114个等位基因，平均每个位点有16.3个等位基因。计算匹配系数后用邻接法进行聚类，结果表明，澳洲指橘与柑橘属的亲缘关系很近；SSR位点的高纯合频率支持富民枳种的地位；枳与柑橘属的关系较远，枳不大可能是从柑橘属衍生而来；Swingle的亚属的划分以及田中的原生柑橘类和后生柑橘类的划分界线不清晰；现代栽培柑橘的起源与大翼橙关系密切；柑橘属的枸橼、柚和宽皮橘都很好地分离，支持其为现代栽培柑橘的3个基本种的观点。
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| 关 键 词： | SSR标记 柑橘属 近缘属植物 亲缘关系 富民枳 澳洲指橘属 |
Phylogenetic Relationships Among Citrus and Its Relatives as Revealed by SSR Markers |
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| Abstract.Phylogenetic Relationships Among Citrus and Its Relatives as Revealed by SSR Markers[J].Journal of Genetics and Genomics,2003,30(1):81-87. |
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| Authors: | Abstract |
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| Abstract: | Phylogenetic relationships among 29 accessions belonging to Citrus,Poncirus,Fortunella,Microcitrus,Eremocitrus,Atlantia and Severinia were investigated using SSR markers.Seven SSR primers generated 114 polymorphic alleles,with an average of 16 3 alleles per primer.Cluster analysis via neighbour-joining method showed that Microcitrus was close to Citrus;Poncirus was distant from Citrus,which suggested that Poncirus could not be derived from Citrus.High frequency of the homozygous SSR locus supported the species status of Fumin trifoliate orange.Seperation of neither Papeda and Citrus nor Archicitrus and Metacitrus was well resolved.The present work confirmed citron,pummelo and mandarin as basic species of cultivated citrus since they could be placed into three distinct clusters. |
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| Keywords: | citrus fumin trifoliate orange microcitrus SSR phylogenetic relationship |
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