居群遗传结构研究中显性标记数据方法初探

为对比显性标记应用于居群遗传结构研究时不同统计参数的适用性，利用RAPD技术对中国5个居群的100个疣粒野生稻个体进行了遗传结构分析。在衡量居群遗传多样性水平时，多态位点比率（PPB）会低估遗传变异的量，其价值不如Shannon多样性指数和Nei基因多样性指数，而采用Nei指数时不必进行Lynch-Milligan矫正。对个体间遗传关系进行分析时，17种遗传相似性指数矩阵两两之间的Mantel检测都表现出极显著的相关性（r＞0.95,t＞t

Analyses of Population Genetic Structure by Using Dominant Markers

Dominant markers tend to under-estimate the amount of geneticdiversity relative to codominant systems when applying in population genetics. In order to compare various existing methods for analysis of genetic structure, RAPD markers were used to detect genetic variability of 5 populations of Oryza granulata from China.The results indicated that both Shannon index of diversity and Nei gene diversity were superior to percentage of polymorphic bands (PPB) because the latter lacked of ability to describe frequency difference of polymorphic bands. Mantel test showed significant relation (r＞0.95, t＞t0.01) among matrice of 17 different genetic similarities, which indicated that all of them could be used in analysis of genetic relations of individuals of Oryza granulata. Both AMOVA analysis based on st distance and analysis of Nei’s distance showed consistent results in defining relationship among the 5 populations, and Lynch-Milligan pruning should be used to improve the estimation of population parameters. All of AMOVA, Gst and Shannon diversity analyses obtained similar results with majority of genetic variation occurring between Yunnan and Hainan, and low levels of genetic diversity resided within regions and populations.