| grp75对细胞缺糖损伤的保护作用 |
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| 引用本文： | 郑东航,左伋,杨增杰,夏蓓莉,张咸宁.grp75对细胞缺糖损伤的保护作用[J].遗传学报,2000,27(8):666-671. |
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| 作者姓名： | 郑东航 左伋 杨增杰 夏蓓莉 张咸宁 |
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| 作者单位： | 上海医科大学遗传医学中心，上海 200032 |
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| 基金项目： | 国家自然科学基金（39670239）和美国中华医学基金（96-635） |
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| 摘 要： | 为研究ｇｒｐ７５的功能，对过表达ｇｒｐ７５的ＣＨＬ细胞进行了无糖培养以施加能量代谢应激，运用台盼蓝染色计数、ＬＤＨ释放测定和流式细胞术等方法评估其损伤程序。结果显示，无糖培养５ｈ，过表达ｇｒｐ７５细胞和对照组细胞比较，细胞活率、亚二倍体细胞率均无明显差别；无糖培养１０ｈ，过表达ｇｒｐ７５细胞的活率高于对照组（Ｐ〈０．０１），亚二倍体细胞率低于对照组（Ｐ〈０．０５）；无糖培养至２０ｈ，两组细胞活率和
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| 关 键 词： | 葡萄糖调节蛋白75 过表达 细胞保护 缺糖损伤 |
| 文章编号： | 0379-4172（08）-666-06 |
| 修稿时间： | 1999年9月15日 |
grp75 Protects Cells from Injuries Caused by Glucose Deprivation |
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| ZHENG Dong-Hang,ZUO Ji,YANG Zeng-Jie,XIA Bei-Li,ZHANG Xian-Ning.grp75 Protects Cells from Injuries Caused by Glucose Deprivation[J].Journal of Genetics and Genomics,2000,27(8):666-671. |
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| Authors: | ZHENG Dong-Hang ZUO Ji YANG Zeng-Jie XIA Bei-Li ZHANG Xian-Ning |
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| Abstract: | To elucidate the functions of grp75, CHL cells over-expressing grp75 are cullled in glucose-free medium in order to simulate energy metabolic stress. Their susceptibilities to injuries caused by glucose deprivation are assessed by trypan blue exclusion, LDH leakage measurement and cytometry analysis. Data shows a stronger resistance to glucose deprivation among cells over-expressing grp75 than among cells constitutively expressing grp75. The outcome suggests that grp75 can protect cells from injuries caused by glucose deprivation. |
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| Keywords: | glucose-regulated proteins 75(grp75) over-expression cell protection |
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