喉癌中p15、p16基因纯合缺失与EGFR基因扩增相关性研究

研究p15、p16基因缺失和EGFR基因扩增的相关性及其与喉癌发生、发展的关系。提取喉癌新鲜组织中基因组DNA，采用聚合酶链反应技术，分别对30例喉癌进行p15基因第2外显子(p15E2)和p16基因第2外显子(p16E2)进行纯合缺失研究；应用FISH方法进行喉癌实体瘤EGFR基因扩增研究。p15E2纯合缺失率为13．3％(4／30)，p16E2纯合缺失率为16．7％(5／30)，p15E2、p16E2共同缺失率为6．7％(2／30)。在30例喉癌实体瘤EGFR基因扩增频率为30％(9／30)，扩增2～8倍。p15E2和p16E2纯合缺失以及二者共同缺失与EGFR基因扩增相关，可能引起细胞周期失控而导致细胞增殖紊乱，在喉癌的发生及恶性进展中发挥一定作用。

Association of Homozygous Deletion of p15 and p16 Gene and Amplification of EGFR Gene in Laryngeal Squamous Cell Carcinoma

To assess the relationship of deletion of p15 and p16 gene and EGFR gene amplification in laryngeal squamous cell carcinoma(LSCC).DNA was extracted from fresh tumor.Deletion of p15 exon 2(p15E2) and p16 exon 2(p16E2) in 30 cases of LSCC was detected by polymerase chain reaction (PCR) technique.Amplification of EGFR gene in 30 cases of LSCC was detected by FISH.The rate of p15E2 deletion in 30 cases was 13.3(4/30),and that of p16E2 was 16.7%(5/30).p15E2 and p16E2 codeletion rate was 6.7%(2/30).The rate of EGFR gene amplification in 30 cases was 30%(9/30),and was amplified 2 to 8 fold.Homozygous deletion of p16E2 and p15E2 and codeletion is related with amplification of EGFR gene(P=0.000018),and may play an important role to oncogenesis and malignant progression in LSCC.