| 硅纳米颗粒作为基因转染载体的研究 |
| |
| 引用本文： | 薛志刚,郑多,阮建明,潘乾,赵迪诚,刘小平,陈玉祥,夏家辉,夏昆.硅纳米颗粒作为基因转染载体的研究[J].遗传学报,2003,30(7):606-610. |
| |
| 作者姓名： | 薛志刚 郑多 阮建明 潘乾 赵迪诚 刘小平 陈玉祥 夏家辉 夏昆 |
| |
| 作者单位： | 1. 中南大学医学遗传学国家重点实验室,长沙,410078
2. 中南大学粉末冶金国家重点实验室,长沙,410083 |
| |
| 基金项目： | 国家自然科学基金资助 (编号 :3 983 0 2 0 0 )~~ |
| |
| 摘 要： | 通过不同浓度的NaCl、NaI修饰硅纳米颗粒，用琼脂糖凝胶电泳分析硅纳米颗粒与DNA结合力及对DNA的保护作用，同时用绿色荧光蛋白基因作报告基因，以硅纳米颗粒作为基因转染的载体，转染HT1080细胞。经电镜观察证实硅纳米颗粒进入细胞内；硅纳米颗粒与DNA结合后，能对DNA起保护作用；并且硅颗粒作为基因转染的载体，将绿色荧光蛋白基因导入HT1080细胞，用荧光显微镜观察到发绿色荧光的细胞。结果表明，硅纳米颗粒可作为基因转染的载体。
|
| 关 键 词： | 硅纳米颗粒 氯化纳 基因转染 载体 |
| 文章编号： | 0379-4172(2003)07-0606-05 |
| 修稿时间： | 2002年8月22日 |
Silica Nanoparticles Modified as Carriers for Gene Transfection |
| |
| XUE Zhi Gang ,ZHENG Duo ,RUAN Jian Ming ,PAN Qian ,ZHAO Di Cheng ,LIU Xiao Ping ,CHEN Yu Xiang ,XIA Jia Hui ,XIA Kun.Silica Nanoparticles Modified as Carriers for Gene Transfection[J].Journal of Genetics and Genomics,2003,30(7):606-610. |
| |
| Authors: | XUE Zhi Gang ZHENG Duo RUAN Jian Ming PAN Qian ZHAO Di Cheng LIU Xiao Ping CHEN Yu Xiang XIA Jia Hui XIA Kun |
| |
| Institution: | XUE Zhi Gang 1,ZHENG Duo 1,RUAN Jian Ming 2,PAN Qian 1,ZHAO Di Cheng 1,LIU Xiao Ping 1,CHEN Yu Xiang 1,XIA Jia Hui 1,XIA Kun 1 |
| |
| Abstract: | The silica nanoparticles are modified by sodium iodine or sodium chloride with different concentration.Their ability to bind and protect plasmid DNA was demonstrated by agarose gel electrophoresis.The EGFP N1 plasmid was transfected into HT1080 by combing silica nanoparticle.Eletromicroscope examine revealed that nanoparticle DNA could enter into cells,these silica nanoparticles protect DNA against nuclease digestion and the GFP has an effective expression.In conclusion,these nanoparticles might be used as DNA carriers for gene transfection. |
| |
| Keywords: | silica nanoparticle sodium chloride gene transfection carrier |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
