蓖麻蚕核型多角体病毒多角体蛋白基因的克隆和核苷酸序列分析

蓖麻蚕(Attacus ricini)是我国特有蚕种,以其核多角体病毒(ArNPV)为载体有可能发展成为新的基因工程表达系统,我们建立了ArNPV基因库,并亚克隆了含多角体蛋白(Ph)基因DNA片段。对该1.1kb全长DNA片段进行序列分析,确定ArNPV Ph结构基因全长735bp,与苜蓿尺蠖NPV(AcNPV)、家蚕NPV(BmNPV)同源性分别为76%和81%,ArNPV 5'端调控结构Rohrmann box与各类NPV的Ph基因相似,但3'下游序列几无同源,显示了ArNPV Ph基因结构的特征性。同时,我们还对Ph基因启动子的其它结构特点作了剖析。

Cloning and Sequencing of Attacus Ricini Nuclear Polyhedrosis Virus Polyhedrin Gene

Attacus ricini is a species of insect which only grows in China. It is expected to construct a new expression system for genetic engineering by using ArNPV as a vector. We have established an ArNPV gene library. The 1.1 kb DNA fragment containing ArNPV Ph gene was subcloned. Sequencing analysis showes that the 735 bp Ph structural gene has the homology of 76% and 81% with those of AcNPV and BmNPV respectively. The Rohrmann box in the ArNPV 5'-end regulation region is very similar to those of various other NPVs, but the 3'-end downstream sequence has almost no homology with those of AcNPV and BmNPV, demonstrating the characteristic property of the structure of ArNPV Ph gene. Other structural features of the Ph gene promoter are also discussed in this paper.