| 限制酶AluI显带和CA_3/DA/DAPI染色表达的家猪染色体结构异染色质 |
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| 引用本文： | 赵亚力,王喜忠,王子淑,陈文元.限制酶AluI显带和CA_3/DA/DAPI染色表达的家猪染色体结构异染色质[J].遗传学报,1990(5). |
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| 作者姓名： | 赵亚力 王喜忠 王子淑 陈文元 |
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| 作者单位： | 四川大学生物系 成都
(赵亚力，王喜忠，王子淑)，四川大学生物系 成都(陈文元) |
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| 摘 要： | 采用限制酶AluI显带、CA_(?)/DA/DAPI荧光染色和常规C带技术研究了家猪染色体着丝粒结构异染色质,结果表明:着丝粒结构异染色质至少可被区分为3类,并且在染色体组内各有其特异的染色体分布。将家猪染色体DA/DAPI荧光带和限制酶AluI显带与人类染色体比较,发现家猪13—18号端着丝粒染色体显带特征与人染色体1,9、16、Y一致。提示家猪13—18号端着丝粒区结构异染色质存在与人类随体DNA相似的DNA组成。
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| 关 键 词： | 限制性内切酶 显带染色体 结构异染色质 |
Expression of Constitutive Heterochromatin by Alul Restriction Endonuclease Treatment and CA_3/DA/DAPI Staining of Domestic Pig Chromosome |
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| Zhao Yali Wang Xizhong Wang Zishu Chen Wengyuan.Expression of Constitutive Heterochromatin by Alul Restriction Endonuclease Treatment and CA_3/DA/DAPI Staining of Domestic Pig Chromosome[J].Journal of Genetics and Genomics,1990(5). |
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| Authors: | Zhao Yali Wang Xizhong Wang Zishu Chen Wengyuan |
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| Abstract: | The heterogeneity of constitutive heterochromatin in two domestic pigs has been studied by restriction endonuclease Alul banding, CA2/DA/DAPI banding and C-banding techniques. The results show that the centromere constitutive heterochromatin can be subdivided into rhree types, which located in different chromosomes. It is found that there is correspondence between No. 13-18 chromosomes in pigs and No. 1,9,16,Y chromosome in human by comparing the banding patterns of Alul and CAs/DA/DAP1. Therefore, it is revealed that satellite DNAs in constitutive heterochromatin of chromosomes mentioned above are similar. |
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| Keywords: | restriction endonuclease ba nding chromosome constitutive heterochromatin |
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