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| 抗甜菜坏死黄脉病毒单链抗体表达载体的构建及其表达 | |
| 引用本文： | 曾君祉,周志勇,吴有强,刘志萍,王文星,黄华梁,蔡祝南,于嘉林.抗甜菜坏死黄脉病毒单链抗体表达载体的构建及其表达[J].遗传学报,2000,27(11):1006-1011. |
| 作者姓名： | 曾君祉 周志勇 吴有强 刘志萍 王文星 黄华梁 蔡祝南 于嘉林 |
| 作者单位： | 1. 中国科学院遗传研究所，北京 100101 2. 东北大学 3. 中国农业大学，北京 100094 |
| 基金项目： | 国家自然科学基金资助(39870408) |
| 摘 要： | 用ＰＣＲ方法以分泌抗甜菜坏死黄脉病毒（ＢＮＹＶＶ）单克隆抗体的杂交瘤细胞的基因组为模板，扩增了编码ＢＮＹＶＶ单抗的重链可变区（ＶＨ）基因。测序表明，该ＶＨ序列属于小鼠ＩＩ（Ａ）亚类，全长为３６０ｂｐ，编码１２０个氨基酸。将其和先前克隆的轻链基因分别插入到一个含有连接ＶＨ和ＶＬ基因的连接序列的质粒之中，构建成单链抗体（ｓｃＦｖ）基因的表达载体ｐＴＣｓｃＦｖ。将质粒在大肠杆菌中表达，ＥＬＩＳＡ法检测出 |
| 关 键 词： | BNYVV 单链抗体 大肠杆菌表达系统 抗病育种 |
| 文章编号： | 0379-4172（2000）11-1006-06 |
| 修稿时间： | 2000年4月11日 |
Expression of Single-chain Fv Antibody for Anti-beet Necrotic Yellow Vein Virus in Escherichia coli |
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| ZENG Jun-Zhi,ZHOU Zhi-Yong,WU You-Qiang,LIU Zhi-Ping,WANG Wen-xing,HUANG Hua-liang,CAI Zhu-Nan,YU Jia-lin.Expression of Single-chain Fv Antibody for Anti-beet Necrotic Yellow Vein Virus in Escherichia coli[J].Journal of Genetics and Genomics,2000,27(11):1006-1011. | |
| Authors: | ZENG Jun-Zhi ZHOU Zhi-Yong WU You-Qiang LIU Zhi-Ping WANG Wen-xing HUANG Hua-liang CAI Zhu-Nan YU Jia-lin |
| Abstract: | The heavy chains variable region gene (VH) of monoclonal antibody against beet necrotic yellow vein virus (BNYVV) was amplified from total DNA extracted from anti-BNYVV hybridoma cells by PCR. Sequencing showed that the VH belongs to mouse subgroup II(A) and contains 360bp, which code one hundred and twenty amino acids. The VH and VL genes were inserted into a plasmid which contains a linker sequence for constructing scFv gene. The new vector named pTC scFv. The scFv was produced in Escherichia coli and appeared binding activity with BNYVV antigen by ELISA method. |
| Keywords: | BNYVV hybridoma single-chain Fv antibody E coli expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |