IgY—Ricin A诱导癌细胞程序化死亡的研究

研究了抗癌导向药物IgY-Ricin A杀伤癌细胞的作用机理。人胃低分化粘液腺癌MGC-803细胞经IgY-Ricin A处理,细胞的增殖明显受到抑制,而同样处理的人胚正常肺细胞2BS,其生长不受这种药物的影响。FCM实验结果表明,IgY-Ricin A处理的MGC-803细胞,在8h开始出现细胞程序化死亡峰Apo,同样处理的2BS细胞则无Apo峰出现。经lgY-Ricin A处理的MGC-803细胞核由均一状态变为浓缩凝集状,经激光共聚焦显微镜进行光切片和三维重组,发现MGC-803细胞核由原来的球形变成高度浓缩凝集的点状结构。DNA凝胶电泳分析显示,IgY-Ricin A处理的MGC-803细胞DNA被降解,呈现梯状电泳条带这一典型的细胞程序化死亡指标。研究结果表明,IgY-Ricin A通过诱导细胞程序化死亡来控制MGC-803细胞的增殖,最终杀死癌细胞。

Studies on Programmed Cell Death of Cancer Induced by IgY-Ricin A

We report here that human stomach cancer MGC-803 cells undergoes apoptosis following exposure to IgY-Ricin A. After the addition of IgY-Ricin A, these cells display typical apoptotic morphological changes: chromatin condensation, cell shrinkage and releasing of apoptotic bodies. More detailed examinations such as flow cytometry and agarose gel electrophoresis also show typical apoptosis hallmarks, respectively. In contrast, the human normal cell 2BS also treated with IgY-Ricin A. It could not induce 2BS apoptosis. These findings strongly suggest that IgY-Ricin A kill cancer cell to pass programmed cell death.