动物细胞系的染色体组型与遗传变异率分析

在建立国内首家犬，猫，猴，鼠传代细胞库，即7种动物肾细胞系（F－81，CRFK，MDCK，Vero,Vero-2,MA-104,BHK-21)的种子库和工作库的基础上，通过细胞染色体组型，G带核型，染色体数目变异率，结构畸变率分析，了解7种细胞系传代培养不同代次的染色体变异情况，以相应的细胞株皮下接种褐 形成肿瘤实验，软琼脂细胞克隆一苦恼经与植物凝集素作用下细胞凝集实验为对照，筛选出无致癌／致瘤性，符合细胞遗传学要求，无传染因子污染的细胞系（F－81，CRFK，Vero,Vero-2)或极低致癌性的MDCK细胞系用于制苗，发现肿瘤细胞系高变异率株可在裸鼠体内快速选育成功，细胞系染色体遗传特征决定致性质并具有种属特异性，得到一些100％成瘤和100％不成瘤的细胞株并了与染色体组型的关系，对于肿瘤的发病机理及实验治疗，都是非常好的模型，一些细胞系不仅成瘤而且还可转移（致恶性横纹肌样瘤的BHK－21和Vero 细胞株），其他致瘤细胞株只成瘤不转移或不明显转移。

Analyses of Chromosomal Karyotypes and Cytogenetic Variations of Animal Cell Lines

After the master cell stock(mcs) and working cell bank of more than 30 different strains of 7 animal kidney cell lines (F-81 or CRFK cell line, MDCK cell line, Vero or Vero-2 cell line, MA-104 cell line and BHK-21 cell line) were established in China, the chromosomal number variations and structural aberrations of the above lines, primary feline or canine kidney cell (FKC or CKC) and HeLa cell line were investigated and their karyotypes of routine or Giemsa chromosomal bands were analyzed. The carcinogenesis or tumorigenicity testing of these cells in about 700 nude mice and for colony formation in soft agar (SA) and for agglutination under different concentration of plant lectins was carried out. Both tumorigenicity-negative strains of F-81, CRFK, Vero or Vero-2 lines and very-low-tumorigenicity strains of MDCK line were successfully selected and evaluated for the production of canine or feline combination viral vaccines, which are free of infectious agents, and described with respect to cytogenetic characteristics and tumorigenicity.