| 小麦抗白粉病基因Pm6的RAPD标记 |
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| 引用本文： | 王心宇,亓增军,马正强,陈佩度,刘大钧.小麦抗白粉病基因Pm6的RAPD标记[J].遗传学报,2000,27(12):1072-1079. |
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| 作者姓名： | 王心宇 亓增军 马正强 陈佩度 刘大钧 |
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| 作者单位： | 南京农业大学农业部作物细胞遗传重点开放实验室，南京 210095 |
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| 基金项目： | 国家“863”计划（Z-17-04-01）资助 |
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| 摘 要： | 从提莫菲维小麦转移到普通小麦中的小麦白粉病抗性基因Ｐｍ６是小麦白粉病（Ｅｒｙｓｉｐｈｅ ｈｒａｍｉｎｉｓ ｆ ｓｐ．ｔｒｉｔｉｃｉ）的有效抗性基因。用７００个随机引物对Ｐｍ６近等基因系进行ＲＡＰＤ分析，发现引物ＯＰＶ２０可在抗病近等基因系中产生大小为２ｋｂ的稳定的多态片段。用该引物检测１０个其他携Ｐｍ６的渐渗系材料，均可稳定扩增出该２ｋｂ的多态片段。理一步用ＯＰＶ２０对Ｐｍ６Ｆ２（ＩＧＶ１－４６３
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| 关 键 词： | 小麦 白粉病 RAPD标记 抗白粉病基因 |
| 修稿时间： | 2000年3月10日 |
Identification of RAPD Markers Tightly Linked to Wheat Powdery Mildew Resistance Gene Pm6 |
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| WANG Xin-Yu,QI Zeng-Jun,MA Zheng-Qiang,CHEN Pei-du,LIU Da-Jun.Identification of RAPD Markers Tightly Linked to Wheat Powdery Mildew Resistance Gene Pm6[J].Journal of Genetics and Genomics,2000,27(12):1072-1079. |
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| Authors: | WANG Xin-Yu QI Zeng-Jun MA Zheng-Qiang CHEN Pei-du LIU Da-Jun |
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| Abstract: | Pm6 transferred from Triticum timopheevii L. to common wheat, is an effective resistance gene to powdery mildew disease caused by Erysiphe graminis f. sp.tritici. The RAPD technique, employing a total of 700 decamer primers, was used to identify polymorphic markers between resistant (IGVI463) and susceptible (Prins) near-isogenic lines. Primer OPV20 produced a 2 000 base pair (bp) reproducible fragment only in the resistant near-isogenic line. The 2 000-bp DNA fragment was present in all other introgression lines containing Pm6. Using the F2 mapping population from a cross of IGVI-463 (PI170914 ／ 7Prins)×Prins, Pm6 was shown to be closely linked to the marker OPV20-2000 at a genetic distance of 3.0±2.2cM. The marker was successfully used in detecting the presence of Pm6 in different genetic backgrounds. |
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| Keywords: | Triticum aestivum T timopheevii powdery mildew introgression lines near-isogenic lines RAPD |
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