云南元江普通野生稻株高和抽穗期QTL定位研究

以云南元江普通野生稻为供体亲本，在特青的遗传背景下构建了一套BC3高代回交群体。利用117个SSR标记分析383个BC3F2株系的基因型，采用单标记分析法对控制元江普野株高和抽穗期的QTL进行分析。在北京和合肥两个地点试验结果表明，控制株高的QTL分布在第1染色体上，在RM104附近有一个QTL，与sd-1位置相当，其对表现型变异的贡献率在两个地点分别为27％和28％，其加性效应值分别为26．24cm和26．28cm，来自野生稻的等位基因显著提高回交群体的株高；在第1、3、7、8、11染色体共检测到6个控制抽穗期QTL，其中第8染色体RM25附近控制抽穗期的QTL在两个地点的贡献率分别为13％和15％，加性效应值为4．60d和3．65d，来自野生稻的等位基因使回交群体抽穗期延迟。

Identification of Quantitative Trait Loci Controlling Plant Height and Days to Heading from Yuanjiang Common Wild Rice( Oryza rufipogon Griff.)

An advanced backcross strategy was used to identify quantitative trait loci (QTLs) associated with plant height and days to heading in the BC_3F_2 population derived from an accession of common wild rice (Oryza rufipogon Griff.) from Yuanjiang in Yunnan Province of China,as the donor,and an elite Indica cultivar "Teqing",as the recipient.Based on analyses of 116 SSR markers distributed throughout the rice genome and by (using) single-point analysis,four putative QTLs derived from O.rufipogon were detected for plant height on chromosome 1,and 6 QTLs for days to heading on chromosome 1,3,7,8 and 11.All Alleles of QTLs for plant height from O.rufipogon could increase plant height of the backcross population.One QTL near RM104 on chromosome 1,explaining 27% and 28% of phenotypic variance and additive value reached 26.24 cm and 26.28 cm respectively in Beijing and Hefei,was detected.The location of this QTL is corresponding to sd-1.One QTL near the marker RM25 on chromosome 1,explaining 13% and 15% of phenotypic variance was identified.The additive value of this QTL reached 4.60 days and 3.65 days respectively in Beijing and Hefei,and the allele from (O.rufipogon could) delay the backcross population's days to heading.