水稻不育系安农S-1育性转换及相关基因的表达分析

通过在自然环境和高温温室内对安农S-1的不同部位进行高温、低温诱导处理，对水稻温敏核不育系安农S-1的温度敏感时期和诱导部位进行了研究。总共进行了8种处理，结果表明：安农S-1的育性转换时期是从花粉母细胞形成到减数分裂的四分体时期之前。在育性转换时期，处于高温的条件下，根部低温处理不能诱导安农S-1可育，穗部低温处理可以使安农S-1保持可育，可见安农S-1的温度敏感部位在幼穗。aprz基因和育性相关，用RT-PCR方法研究了aprt基因在安农S-1不同部位和不同温度环境的表达变化，结果显示，在幼穗中aprt基因的表达在高温环境中被大幅度下调，而在叶中和根中的变化比较小，这说明幼穗对温度最敏感，从侧面验证了引起安农S-1育性转换的温度敏感部位是在幼穗。

Fertility Alteration of Male Sterile Rice Line Annong S-1 and the Expression of Fertility Related aprt Gene

The thermo-sensitive point and the thermo-sensitive stage for fertility alteration in the thermo-sensitive genic male sterile (TGMS) line Annong S-1 were studied.In nature environment and green house,the leaf,root and young panicle were treated by low and high temperature respectively.In total,8 treatments were carried out.Results indicated that during the thermo-sensitive stage of fertility alteration,in high temperature environmental conditions,the low temperature treatment in the root did not significantly induce Annong S-1 to be fertile,while the low temperature treatment in young panicle obviously induced Annong S-1 to be fertile.Therefore,the thermo-sensitive point of Annong S-1 is the young panicle.The stage of fertility alteration was also investigated.Results indicated that alteration occurred in the phase from the formation of pollen mother cell to the tetrad of miosis.aprt gene is related the male sterility.The expression of aprt gene in root,young panicle and leaf were investigated by RT-PCR respectively.In young panicle,the expression of aprt gene was greatly down regulated by high temperature,while in root and leaf the expressions have no great changes.These data show that the young panicle is sensitive to temperature change.The result also supported that the young panicle is the thermo-sensitive point of Annong S-1 in the fertility alteration.These conclusions can be used to direct in hybrid rice seed production.