猪肌肉蛋白质含量的QTL分析

用酶山猪与大白猪构建的资源家系检测影响肌肉蛋白质含量的数量性状座位（QTL）。测定135头F2代个体及其相应父母和祖父母在分布1号、2号和6号染色体上的24个微卫星基因型。用这些微卫星标记计算每个F2代个体固定位置的加快和显性系数，以1cM的间距肌肉蛋白质含量与这些系数进行回归分析，发现3个与肌肉蛋白质含量具有显著效应的QTL（P＜0．05），它们分别于位于1号染色体的Sw1332和Sw2130之间，2号染色体的Sw2516和Sw1201之间，6号染色体的Sw2516和Sw1201之间，分别解释0．91％、12．76％和4．60％的肌肉蛋白质含量变异方差。结果提示，影肌肉蛋白质含量的QTL分布在多条染色体上。

Detection of Quantitative Trait Loci for Protein Percentage in Muscle in Resource Pig Population

An intercross between Meishan and Large White pig population was used to map quantitative trait loci(QTL) for protein percentage in muscle. In the present study,all 135 F 2 animals,their parents and their grandparents were genotyped for 24 microsatellites,which were in 1,2 and 6 chromosome. The marker genotypes were used to calculate the additive and dominant coefficients at fixed position in the genome of each F 2 animal,and the protein percentage were regressed on to the coefficients in intervals of 1cM. Three significant QTLs effects were found for protein percentage in muscle. They were located between markers Sw1332 and Sw2130 at chromosome 1,between Sw2516 and Sw1201 at chromosome 2 and between Sw322 and Sw607 at chromosome 6( P <0.05),and the explained residual variance were 0.91%,12.76% and 4.60%,respectively. The results show that QTLs,which affect protein percentage,may be located in different chromosomes.